SHOX Polyclonal antibody

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  WB analysis of mouse brain using 25117-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25117-1-AP (SHOX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 25117-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25117-1-AP (SHOX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SHOX Polyclonal Antibody for WB, ELISA

Cat No. 25117-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

GCFX, PHOG, short stature homeobox, Short stature homeobox protein, SHOX, SHOXY, SS

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Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25117-1-AP targets SHOX in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHOX fusion protein Ag18930
• Full Name: short stature homeobox
• Calculated Molecular Weight: 292 aa, 32 kDa
• Observed Molecular Weight: 25-32 kDa
• GenBank Accession Number: BC148783
• Gene Symbol: SHOX
• Gene ID (NCBI): 6473
• RRID: AB_2879905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15266
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Short stature homeobox protein (SHOX), also named as GCFX, is a 292 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. SHOX. SHOX controls fundamental aspects of growth and development. SHOX undergoes splicing resulting in two isoforms, SHOXA and SHOXB. SHOXA is a 32 kDa protein and is expressed in skeletal muscle, placental, pancreas, heart and bone marrow fibroblast and, to a lesser extent, in kidney and lung. SHOXB is 25 kDa protein and is highly expressed in osteogenic cells, but not expressed in brain, kidney, liver or lung.