SIK2 Polyclonal antibody

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  WB analysis using 27608-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27608-1-AP (SIK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27608-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27608-1-AP (SIK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SIK2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27608-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

SIK 2, salt inducible kinase 2, Qin induced kinase, QIK, LOH11CR1I

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Tested Applications

• Positive WB detected in: SKOV-3 cells, U2OS cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27608-1-AP targets SIK2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIK2 fusion protein Ag26468
• Full Name: salt-inducible kinase 2
• Calculated Molecular Weight: 926 aa, 104 kDa
• Observed Molecular Weight: 103-110 kDa
• GenBank Accession Number: BC113459
• Gene Symbol: SIK2
• Gene ID (NCBI): 23235
• RRID: AB_3669614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H0K1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Eur J Pharmacol; The anti-rotavirus effect of baicalin via the gluconeogenesis-related p-JNK-PDK1-AKT-SIK2 signaling pathway.; Authors - Lijun Song