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SIP1 antibody (mAb) (Clone 6E5)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ICC, IF, IHC, WB

CloneNo.

6E5

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Cat No : 61095,61096 61095

Synonyms

SIP1, Smad-interacting protein 1, ZEB2, Zinc finger E-box-binding homeobox 2, sequence-specific DNA binding protein, transcriptional repressor, embryonic development of neural structures, neural crest. delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain, SMAD1, SMAD2, SMAD3, CBX4, CTBP1, Hirschsprung disease, HSCR, clone 6E5, monoclonal, sample, dbdabs

Validation Data Gallery

  • SIP1 antibody (mAb) tested by Western blot. Detection of SIP1 by Western blot. The analysis was performed using 20 ug of Saos-2 nuclear cell extract and SIP1 (mAb) at a 1 ug/ml dilution.

    SIP1 antibody (mAb) tested by Western blot. Detection of SIP1 by Western blot. The analysis was performed using 20 ug of Saos-2 nuclear cell extract and SIP1 (mAb) at a 1 ug/ml dilution.

Product Information

Tested Applications ICC, IF, IHC, WB

Applications Validated by Active Motif: WB: 1 - 4 ug/ml dilution
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen This SIP1 antibody was raised against a recombinant protein corresponding to amino acids 1-360 of human SIP1.
Full Name SIP1 antibody (mAb) (Clone 6E5)
Synonyms SIP1, Smad-interacting protein 1, ZEB2, Zinc finger E-box-binding homeobox 2, sequence-specific DNA binding protein, transcriptional repressor, embryonic development of neural structures, neural crest. delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain, SMAD1, SMAD2, SMAD3, CBX4, CTBP1, Hirschsprung disease, HSCR, clone 6E5, monoclonal, sample, dbdabs
Molecular weight 180 kDa
GenBank accession numberNP_055610
RRIDAB_2614989
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

SIP1 (Smad-interacting protein 1, aka ZEB2, Zinc finger E-box-binding homeobox 2) is a sequence-specific DNA binding protein and transcriptional repressor that is involved in normal embryonic development of neural structures and the neural crest. SIP1 belongs to the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins and contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1 interacts directly with activated forms of SMAD1, SMAD2 and SMAD3, as well as CBX4 and CTBP1. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR).