SIP1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Cat No : 61113,61114

Synonyms

SIP1, Smad-interacting protein 1, ZEB2, Zinc finger E-box-binding homeobox 2, sequence-specific DNA binding protein, transcriptional repressor, embryonic development of neural structures, neural crest, delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain, SMAD1, SMAD2, SMAD3, CBX4, CTBP1, Hirschsprung disease, HSCR, sample, dbdabs

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Validation Data Gallery

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  SIP1 antibody (pAb) tested by Western blot Nuclear extract of Saos-2 cells (20 ug per lane) probed with SIP1 antibody (pAb) at a dilution of 1:500.

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Product Information

• Tested Applications: WB

  Applications Validated by Active Motif: WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This SIP1 antibody was raised against a peptide from the N-terminus of human SIP1.
• Full Name: SIP1 antibody (pAb)
• Synonyms: SIP1, Smad-interacting protein 1, ZEB2, Zinc finger E-box-binding homeobox 2, sequence-specific DNA binding protein, transcriptional repressor, embryonic development of neural structures, neural crest, delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain, SMAD1, SMAD2, SMAD3, CBX4, CTBP1, Hirschsprung disease, HSCR, sample, dbdabs
• Molecular weight: 180 kDa
• GenBank accession number: NP_055610
• RRID: AB_2793511
• Purification Method: Affinity Purified
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

SIP1 (Smad-interacting protein 1, aka ZEB2, Zinc finger E-box-binding homeobox 2) is a sequence-specific DNA binding protein and transcriptional repressor that is involved in normal embryonic development of neural structures and the neural crest. SIP1 belongs to the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins and contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1 interacts directly with activated forms of SMAD1, SMAD2 and SMAD3, as well as CBX4 and CTBP1. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR).