• Featured Product
  • KD/KO Validated

SIRT2-Specific Polyclonal antibody

SIRT2-Specific Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 19655-1-AP

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2



Tested Applications

Positive WB detected inrat brain tissue, human brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19655-1-AP is SIRT2-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity bovine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRT2-Specific fusion protein Ag7756
Full Name sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Calculated molecular weight 43 kDa
Observed molecular weight 39-47 kDa
GenBank accession numberBC003547
Gene symbol SIRT2
Gene ID (NCBI) 22933
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (10545947). SirT2, a mammalian homolog of Sir2, deacetylates α-tubulin at Lys40 and histone H4 at Lys16 and has been implicated in cytoskeletal regulation and progression through mitosis (12620231,16648462). SirT2 protein is mainly cytoplasmic and is associated with microtubules and HDAC6, another tubulin deacetylase (12620231). Deacetylation of α-tubulin decreases its stability and may be required for proper regulation of cell shape, intracellular transport, cell motility, and cell division (12620231,10966460). The abundance and phosphorylation state of SirT2 increase at the G2/M transition of the cell cycle, and SirT2 relocalizes to chromatin during mitosis when histone H4 Lys16 acetylation levels decrease (16648462,12697818). Overexpression of SirT2 prolongs mitosis, while overexpression of the CDC14B phosphatase results in both decreased phosphorylation and abundance of SirT2, allowing for proper mitotic exit (12697818). Thus, the deacetylation of both histone H4 and α-tubulin by SirT2 may be critical for proper chromatin and cytoskeletal dynamics required for completion of mitosis. This antibody recognizes the 37-45 KD SIRT2 proteins. This antibody is a specific antibody that it can't detect signal with SIRT2-KO samples.

Protocols

Product Specific Protocols
WB protocol for SIRT2-Specific antibody 19655-1-APDownload protocol
IHC protocol for SIRT2-Specific antibody 19655-1-APDownload protocol
IP protocol for SIRT2-Specific antibody 19655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Development

Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics.

Authors - Hong-Hui Wang
bovineWB,IHC,IF

Int J Mol Sci

SIRT2 Inhibition Results in Meiotic Arrest, Mitochondrial Dysfunction, and Disturbance of Redox Homeostasis during Bovine Oocyte Maturation.

Authors - Dejun Xu
ratWB

Small

Key Role of Microtubule and Its Acetylation in a Zinc Oxide Nanoparticle-Mediated Lysosome-Autophagy System.

Authors - Jia Liu
mouseWB

Mol Hum Reprod

RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis.

Authors - Yu Zhang
humanIHC

Front Genet

Comprehensive Analysis of Expression and Prognostic Value of Sirtuins in Ovarian Cancer.

Authors - Xiaodan Sun
mouseWB

Front Oncol

Context-Dependent Roles for SIRT2 and SIRT3 in Tumor Development Upon Calorie Restriction or High Fat Diet.

Authors - Mohamed A Ahmed