Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | rat brain tissue, human skeletal muscle tissue, mouse brain tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19655-1-AP targets SIRT2 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, zebrafish, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7756 Product name: Recombinant human SIRT2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-352 aa of BC003547 Sequence: MDFLRNLFSQTLSLGSQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDFRSPSTGLYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYFMRLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPFLGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQSGAGVPNPSTSASPKKSPPPAKDEARTTEREKPQ Predict reactive species |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 37-45 kDa |
| GenBank Accession Number | BC003547 |
| Gene Symbol | SIRT2 |
| Gene ID (NCBI) | 22933 |
| RRID | AB_2878592 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IXJ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (10545947). SirT2, a mammalian homolog of Sir2, deacetylates α-tubulin at Lys40 and histone H4 at Lys16 and has been implicated in cytoskeletal regulation and progression through mitosis (12620231,16648462). SirT2 protein is mainly cytoplasmic and is associated with microtubules and HDAC6, another tubulin deacetylase (12620231). Deacetylation of α-tubulin decreases its stability and may be required for proper regulation of cell shape, intracellular transport, cell motility, and cell division (12620231,10966460). The abundance and phosphorylation state of SirT2 increase at the G2/M transition of the cell cycle, and SirT2 relocalizes to chromatin during mitosis when histone H4 Lys16 acetylation levels decrease (16648462,12697818). Overexpression of SirT2 prolongs mitosis, while overexpression of the CDC14B phosphatase results in both decreased phosphorylation and abundance of SirT2, allowing for proper mitotic exit (12697818). Thus, the deacetylation of both histone H4 and α-tubulin by SirT2 may be critical for proper chromatin and cytoskeletal dynamics required for completion of mitosis. This antibody recognizes the 37-45 KD SIRT2 proteins. This antibody is a specific antibody that it can't detect signal with SIRT2-KO samples.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SIRT2 antibody 19655-1-AP | Download protocol |
| IP protocol for SIRT2 antibody 19655-1-AP | Download protocol |
| WB protocol for SIRT2 antibody 19655-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SIRT2 Polyclonal Antibody (Cat# 19655-1-AP) has accumulated 98 citations across high-impact journals including Nature Aging, Nature Communications, Cell Metabolism, Neuron, Science Translational Medicine, Journal of Clinical Investigation, Advanced Science, Oncogene, Cell Reports, and Free Radical Biology & Medicine. Associated research fields span sirtuin biology, post-translational modifications, cancer, neuroscience, cardiology, metabolism, immunology, reproductive biology, and aging.
| Species | Application | Title |
|---|---|---|
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Nat Aging SIRT2 counteracts primate cardiac aging via deacetylation of STAT3 that silences CDKN2B
| ||
Cell Mol Immunol GITR exacerbates lysophosphatidylcholine-induced macrophage pyroptosis in sepsis via posttranslational regulation of NLRP3 | ||
Cell Mol Immunol A metabolite from commensal Candida albicans enhances the bactericidal activity of macrophages and protects against sepsis | ||
Nat Commun N6-methyladenosine of Spi2a attenuates inflammation and sepsis-associated myocardial dysfunction in mice | ||
Reviews
What customers say
One reviewer tested this antibody for Western Blot at a 1:200 dilution on sheep atrial homogenates and reported no detectable band. The product arrived quickly, and the reviewer praised Proteintech's responsive customer support for offering assistance with the issue. Overall, the experience was mixed: while the technical performance on the tested tissue was unsatisfactory, the service and shipping were well received.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alice (Verified Customer) (09-23-2025) | *previous review had wrong LOT no.* This antibody arrived fast but produced no band with sheep atrial homogenates. The friendly reps at proteintech said that if I contact the company, they'll try to help. This is great service & I've had no other problems with other antibodies from Proteintech!
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