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- KD/KO Validated
SIRT2 Polyclonal antibody
SIRT2 Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 19655-1-AP
Validation Data Gallery
|Positive WB detected in||rat brain tissue, human brain tissue, mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human heart tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19655-1-AP is SIRT2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SIRT2 fusion protein Ag7756|
|Full Name||sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||39-47 kDa|
|GenBank accession number||BC003547|
|Gene ID (NCBI)||22933|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (10545947). SirT2, a mammalian homolog of Sir2, deacetylates α-tubulin at Lys40 and histone H4 at Lys16 and has been implicated in cytoskeletal regulation and progression through mitosis (12620231,16648462). SirT2 protein is mainly cytoplasmic and is associated with microtubules and HDAC6, another tubulin deacetylase (12620231). Deacetylation of α-tubulin decreases its stability and may be required for proper regulation of cell shape, intracellular transport, cell motility, and cell division (12620231,10966460). The abundance and phosphorylation state of SirT2 increase at the G2/M transition of the cell cycle, and SirT2 relocalizes to chromatin during mitosis when histone H4 Lys16 acetylation levels decrease (16648462,12697818). Overexpression of SirT2 prolongs mitosis, while overexpression of the CDC14B phosphatase results in both decreased phosphorylation and abundance of SirT2, allowing for proper mitotic exit (12697818). Thus, the deacetylation of both histone H4 and α-tubulin by SirT2 may be critical for proper chromatin and cytoskeletal dynamics required for completion of mitosis. This antibody recognizes the 37-45 KD SIRT2 proteins. This antibody is a specific antibody that it can't detect signal with SIRT2-KO samples.
Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics.
Int J Mol Sci
SIRT2 Inhibition Results in Meiotic Arrest, Mitochondrial Dysfunction, and Disturbance of Redox Homeostasis during Bovine Oocyte Maturation.
Key Role of Microtubule and Its Acetylation in a Zinc Oxide Nanoparticle-Mediated Lysosome-Autophagy System.
Mol Hum Reprod
RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis.
Comprehensive Analysis of Expression and Prognostic Value of Sirtuins in Ovarian Cancer.
Context-Dependent Roles for SIRT2 and SIRT3 in Tumor Development Upon Calorie Restriction or High Fat Diet.