• Featured Product
  • KD/KO Validated

SIRT5 Polyclonal antibody

SIRT5 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15122-1-AP

Synonyms

SIR2 like protein 5, SIR2L5, SIRT5



Tested Applications

Positive WB detected inHeLa cells, HCT 116 cells, RAW 264.7 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15122-1-AP targets SIRT5 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRT5 fusion protein Ag7195
Full Name sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
Calculated molecular weight 34 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000126
Gene symbol SIRT5
Gene ID (NCBI) 23408
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.

Protocols

Product Specific Protocols
WB protocol for SIRT5 antibody 15122-1-APDownload protocol
IF protocol for SIRT5 antibody 15122-1-APDownload protocol
IP protocol for SIRT5 antibody 15122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
mouseWB

Nat Commun

Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.

Authors - Min Liu
mouseWB

Diabetes

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Authors - Hua Qu
  • KD Validated
mouseWB,IF,IHC

EBioMedicine

SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice.

Authors - Yipeng Du
humanWB,IF

Mol Oncol

SIRT5-mediated deacetylation of LDHB promotes autophagy and tumorigenesis in colorectal cancer.

Authors - Liang Shi
  • KD Validated
mouseWB

Cell Prolif

20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.

Authors - Wenxing Cui