SIRT5 Polyclonal antibody

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  WB analysis using 15122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15122-1-AP (SIRT5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15122-1-AP

  IP result of anti-SIRT5 (IP:15122-1-AP, 4ug; Detection:15122-1-AP 1:500) with mouse liver tissue lysate 6000ug.

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  IF Staining of HeLa using 15122-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15122-1-AP (SIRT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

SIRT5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 15122-1-AP

Publications(53)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

SIR2-like protein 5, SIR2L5, Regulatory protein SIR2 homolog 5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, EC:2.3.1.-

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Tested Applications

• Positive WB detected in: HCT 116 cells, RAW 264.7 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 50 publications below
• IHC: See 10 publications below
• IF: See 8 publications below
• IP: See 3 publications below
• CoIP: See 4 publications below

Product Information

15122-1-AP targets SIRT5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT5 fusion protein Ag7195
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC000126
• Gene Symbol: SIRT5
• Gene ID (NCBI): 23408
• RRID: AB_2188778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NXA8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
mouse	WB,IHC	Adv Sci (Weinh); Sirtuin 5-Mediated Desuccinylation of ALDH2 Alleviates Mitochondrial Oxidative Stress Following Acetaminophen-Induced Acute Liver Injury; Authors - Qiwen Yu
rat	WB,IF,CoIP	Exp Mol Med; SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis; Authors - Jianxin Mao ; KD Validated
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
human	WB	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma ; KD Validated