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SIRT5 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 15122-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

SIRT5 Antibody WB HeLa cells 15122-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 15122-1-AP(SIRT5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

SIRT5 Antibody IP mouse liver tissue 15122-1-AP

IP Result of anti-SIRT5 (IP:15122-1-AP, 4ug; Detection:15122-1-AP 1:500) with mouse liver tissue lysate 6000ug.

SIRT5 Antibody IF HeLa cells 15122-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 15122-1-AP(SIRT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, mouse liver tissue
Positive IP detected in:
mouse liver tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SIRT5 fusion protein Ag7195
Full name:
sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
Calculated molecular weight:
34 kDa
Observed molecular weight:
33 kDa
GenBank accession number:
BC000126
Gene ID (NCBI):
23408
Gene symbol
SIRT5
Synonyms
SIR2 like protein 5, SIR2L5, SIRT5

Background

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.


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