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SIRT5 Polyclonal antibody
SIRT5 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15122-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HCT 116 cells, RAW 264.7 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 6 publications below |
WB | See 23 publications below |
IHC | See 4 publications below |
IF | See 5 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
15122-1-AP targets SIRT5 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SIRT5 fusion protein Ag7195 |
Full Name | sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) |
Calculated molecular weight | 34 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC000126 |
Gene symbol | SIRT5 |
Gene ID (NCBI) | 23408 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.
Protocols
Product Specific Protocols | |
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WB protocol for SIRT5 antibody 15122-1-AP | Download protocol |
IF protocol for SIRT5 antibody 15122-1-AP | Download protocol |
IP protocol for SIRT5 antibody 15122-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Nat Commun Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling. | ||
Diabetes Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.
| ||
EBioMedicine SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice. | ||
Mol Oncol SIRT5-mediated deacetylation of LDHB promotes autophagy and tumorigenesis in colorectal cancer.
| ||
Cell Prolif 20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study. |