SIRT5 Polyclonal antibody

• Featured Product
• KD/KO Validated

SIRT5 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 15122-1-AP

Synonyms

SIR2 like protein 5, SIR2L5, SIRT5

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Validation Data Gallery

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  WB analysis of HeLa using 15122-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15122-1-AP (SIRT5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 15122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15122-1-AP (SIRT5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15122-1-AP

  IP Result of anti-SIRT5 (IP:15122-1-AP, 4ug; Detection:15122-1-AP 1:500) with mouse liver tissue lysate 6000ug.

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  IF Staining of HeLa using 15122-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15122-1-AP (SIRT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HCT 116 cells, RAW 264.7 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 23 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

15122-1-AP targets SIRT5 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT5 fusion protein Ag7195
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
• Calculated molecular weight: 34 kDa
• Observed molecular weight: 33 kDa
• GenBank accession number: BC000126
• Gene symbol: SIRT5
• Gene ID (NCBI): 23408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
mouse	WB	Diabetes; Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.; Authors - Hua Qu ; KD Validated
mouse	WB,IF,IHC	EBioMedicine; SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice.; Authors - Yipeng Du
human	WB,IF	Mol Oncol; SIRT5-mediated deacetylation of LDHB promotes autophagy and tumorigenesis in colorectal cancer.; Authors - Liang Shi ; KD Validated
mouse	WB	Cell Prolif; 20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.; Authors - Wenxing Cui