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  • KD/KO Validated

SIRT5 Polyclonal antibody

SIRT5 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15122-1-AP


SIR2 like protein 5, SIR2L5, SIRT5

Tested Applications

Positive WB detected inHCT 116 cells, RAW 264.7 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15122-1-AP targets SIRT5 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRT5 fusion protein Ag7195
Full Name sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
Calculated molecular weight 34 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000126
Gene symbol SIRT5
Gene ID (NCBI) 23408
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.


Product Specific Protocols
WB protocol for SIRT5 antibody 15122-1-APDownload protocol
IF protocol for SIRT5 antibody 15122-1-APDownload protocol
IP protocol for SIRT5 antibody 15122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu

Exp Mol Med

SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis

Authors - Jianxin Mao
  • KD Validated

Nat Commun

Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.

Authors - Min Liu

Free Radic Biol Med

SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.

Authors - Yuanzhen Ma
  • KD Validated


Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Authors - Hua Qu
  • KD Validated

Int J Mol Sci

Magnesium Isoglycyrrhizinate Reduces the Target-Binding Amount of Cisplatin to Mitochondrial DNA and Renal Injury through SIRT3

Authors - Xinyu Wang