|Positive WB detected in||HeLa cells, mouse liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15122-1-AP targets SIRT5 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SIRT5 fusion protein Ag7195|
|Full Name||sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC000126|
|Gene ID (NCBI)||23408|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.
Free Radic Biol Med
SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.
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Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
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Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7.
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Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl-CoA acetyltransferase 1.
J Mol Cell Cardiol
Defining decreased protein succinylation of failing human cardiac myofibrils in ischemic cardiomyopathy.