SIRT5 Recombinant antibody

SIRT5 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse







Cat no : 82246-1-RR


FLJ36950, SIR2 like protein 5, SIR2L5, SIRT5

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, HCT 116 cells, RAW 264.7 cells
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82246-1-RR targets SIRT5 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SIRT5 fusion protein Ag7195
Full Name sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 32-33 kDa
GenBank Accession NumberBC000126
Gene Symbol SIRT5
Gene ID (NCBI) 23408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and this contributes to the regulation of blood ammonia levels.


Product Specific Protocols
WB protocol for SIRT5 antibody 82246-1-RRDownload protocol
IF protocol for SIRT5 antibody 82246-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols