- Featured Product
- KD/KO Validated
SIRT6 Polyclonal antibody
SIRT6 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 13572-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, mouse liver tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 12 publications below |
| IHC | See 7 publications below |
| IF | See 4 publications below |
Product Information
13572-1-AP targets SIRT6 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | bovine, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SIRT6 fusion protein Ag4469 |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 39-45 kDa |
| GenBank accession number | BC028220 |
| Gene symbol | SIRT6 |
| Gene ID (NCBI) | 51548 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889). This antibody is a rabbit polyclonal antibody raised against full length human SIRT6 antigen.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SIRT6 antibody 13572-1-AP | Download protocol |
| IHC protocol for SIRT6 antibody 13572-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Int SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial-mesenchymal transition. | ||
Onco Targets Ther SIRT6 promotes the Warburg effect of papillary thyroid cancer cell BCPAP through reactive oxygen species.
| ||
Connect Tissue Res The effect of intervertebral disc degenerative change on biological characteristics of nucleus pulposus mesenchymal stem cell: an in vitro study in rats. | ||
Biochem Biophys Res Commun Autophagy activated by SIRT6 regulates Aβ induced inflammatory response in RPEs. | ||
Front Genet Comprehensive Analysis of Expression and Prognostic Value of Sirtuins in Ovarian Cancer. | ||
Oncol Lett Associations of sirtuins with clinicopathological variables and prognosis in human ovarian cancer. |
