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  • KD/KO Validated

SIRT6 Polyclonal antibody

SIRT6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13572-1-AP

Synonyms

SIR2 like protein 6, SIR2L6, SIRT6



Tested Applications

Positive WB detected inBxPC-3 cells, mouse liver tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

13572-1-AP targets SIRT6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRT6 fusion protein Ag4469
Full Name sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
Calculated molecular weight 39 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberBC028220
Gene symbol SIRT6
Gene ID (NCBI) 51548
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889). This antibody is a rabbit polyclonal antibody raised against full length human SIRT6 antigen.

Protocols

Product Specific Protocols
WB protocol for SIRT6 antibody 13572-1-APDownload protocol
IHC protocol for SIRT6 antibody 13572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Authors - Hu Lei
humanWB

Nat Commun

Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.

Authors - Ziwen Wang
mouseWB

Redox Biol

A new FGF1 variant protects against adriamycin-induced cardiotoxicity via modulating p53 activity.

Authors - Mengjie Xiao
humanWB,IF

Oncogene

Sirtuin-mediated deacetylation of hnRNP A1 suppresses glycolysis and growth in hepatocellular carcinoma.

Authors - Hao Yang
ratWB,IF

Stem Cells

SIRT6 regulates osteogenic differentiation of rat bone marrow mesenchymal stem cells partially via suppressing the nuclear factor-kappa B signaling pathway.

Authors - Hualing Sun
  • KD Validated
mouseIP

Cell Mol Gastroenterol Hepatol

Hepatic SIRT6 modulates transcriptional activities of FXR to alleviate acetaminophen-induced hepatotoxicity.

Authors - Changhui Liu