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  • KD/KO Validated

SIRT6 Polyclonal antibody

SIRT6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13572-1-AP

Synonyms

SIR2 like protein 6, SIR2L6, SIRT6



Tested Applications

Positive WB detected inBxPC-3 cells, mouse liver tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

13572-1-AP targets SIRT6 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity bovine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRT6 fusion protein Ag4469
Full Name sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
Calculated molecular weight 39 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberBC028220
Gene symbol SIRT6
Gene ID (NCBI) 51548
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889). This antibody is a rabbit polyclonal antibody raised against full length human SIRT6 antigen.

Protocols

Product Specific Protocols
WB protocol for SIRT6 antibody 13572-1-APDownload protocol
IHC protocol for SIRT6 antibody 13572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IHC

Cancer Cell Int

SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial-mesenchymal transition.

Authors - Zhou Yang
humanWB

Onco Targets Ther

SIRT6 promotes the Warburg effect of papillary thyroid cancer cell BCPAP through reactive oxygen species.

Authors - Weiping Yu
  • KD Validated
ratWB

Connect Tissue Res

The effect of intervertebral disc degenerative change on biological characteristics of nucleus pulposus mesenchymal stem cell: an in vitro study in rats.

Authors - Yang Liu
mouseIF

Biochem Biophys Res Commun

Autophagy activated by SIRT6 regulates Aβ induced inflammatory response in RPEs.

Authors - Yiji Feng
humanIHC

Front Genet

Comprehensive Analysis of Expression and Prognostic Value of Sirtuins in Ovarian Cancer.

Authors - Xiaodan Sun
humanIHC

Oncol Lett

Associations of sirtuins with clinicopathological variables and prognosis in human ovarian cancer.

Authors - Qikuan He