SIRT6 Polyclonal antibody

• Featured Product
• KD/KO Validated

SIRT6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, FC, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

41

Cat no : 13572-1-AP

Synonyms

SIR2 like protein 6, SIR2L6, SIRT6

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Validation Data Gallery

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  WB analysis using 13572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13572-1-AP (SIRT6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13572-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13572-1-AP (SIRT6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 13572-1-AP

  IP result of anti-SIRT6 (IP:13572-1-AP, 4ug; Detection:13572-1-AP 1:2000) with BxPC-3 cells lysate 2080 ug.

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  IHC staining of mouse testis using 13572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13572-1-AP (SIRT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13572-1-AP (SIRT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, mouse liver tissue, HepG2 cells, PC-3 cells
• Positive IP detected in: BxPC-3 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 29 publications below
• IHC: See 12 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

13572-1-AP targets SIRT6 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT6 fusion protein Ag4469
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC028220
• Gene Symbol: SIRT6
• Gene ID (NCBI): 51548
• RRID: AB_2188915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889). This antibody is a rabbit polyclonal antibody raised against full length human SIRT6 antigen.

Publications

Species	Application	Title
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
mouse	WB	Redox Biol; A new FGF1 variant protects against adriamycin-induced cardiotoxicity via modulating p53 activity.; Authors - Mengjie Xiao
human	WB,IF	Oncogene; Sirtuin-mediated deacetylation of hnRNP A1 suppresses glycolysis and growth in hepatocellular carcinoma.; Authors - Hao Yang
rat	WB,IF	Stem Cells; SIRT6 regulates osteogenic differentiation of rat bone marrow mesenchymal stem cells partially via suppressing the nuclear factor-kappa B signaling pathway.; Authors - Hualing Sun ; KD Validated
mouse	WB	Oxid Med Cell Longev; Total Sesquiterpene Glycosides from Loquat Leaves Ameliorate HFD-Induced Insulin Resistance by Modulating IRS-1/GLUT4, TRPV1, and SIRT6/Nrf2 Signaling Pathways.; Authors - Ruoyun Wu

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