SIRT6 Monoclonal antibody

SIRT6 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2H4G8

Cat no : 67510-1-Ig

Synonyms

SIR2 like protein 6, SIR2L6, SIRT6



Tested Applications

Positive WB detected inJAR cells, NCCIT cells, HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67510-1-Ig targets SIRT6 in WB,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIRT6 fusion protein Ag24103
Full Name sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
Calculated molecular weight 39 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC028220
Gene symbol SIRT6
Gene ID (NCBI) 51548
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889).

Protocols

Product Specific Protocols
WB protocol for SIRT6 antibody 67510-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Prolif

20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.

Authors - Wenxing Cui