SIRT6 Monoclonal antibody

SIRT6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2H4G8

Cat no : 67510-1-Ig

Synonyms

SIR2 like protein 6, SIR2L6, SIRT6

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Validation Data Gallery

• 

  WB analysis using 67510-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67510-1-Ig (SIRT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: JAR cells, NCCIT cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HL-60 cells.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

67510-1-Ig targets SIRT6 in WB, IF, CoIP, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRT6 fusion protein Ag24103
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC028220
• Gene Symbol: SIRT6
• Gene ID (NCBI): 51548
• RRID: AB_2882731
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889).

Publications

Species	Application	Title
mouse	WB	Cell Prolif; 20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.; Authors - Wenxing Cui
mouse	WB	ACS Omega; Harnessing the Therapeutic Potential of Ginsenoside Rd for Activating SIRT6 in Treating a Mouse Model of Nonalcoholic Fatty Liver Disease; Authors - Tianqi Cui
mouse	IF,WB,CoIP	Mol Med; Microglia Sirt6 modulates the transcriptional activity of NRF2 to ameliorate high-fat diet-induced obesity; Authors - Xiaoxia Xiao
mouse	WB	J Orthop Res; Orientin inhibits inflammation in chondrocytes and attenuates osteoarthritis through Nrf2/NF-κB and SIRT6/NF-κB pathway; Authors - Weiyi Xia