|Positive WB detected in||mouse liver tissue, human kidney tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||rat liver tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12994-1-AP targets SIRT7 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SIRT7 fusion protein Ag3656|
|Full Name||sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)|
|Calculated molecular weight||400 aa, 45 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||BC017305|
|Gene ID (NCBI)||51547|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.
Oxid Med Cell Longev
SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway.
Comprehensive Analysis of Expression and Prognostic Value of Sirtuins in Ovarian Cancer.
Lycorine hydrochloride suppresses stress-induced premature cellular senescence by stabilizing the genome of human cells.
Int J Clin Exp Pathol
High expression of Sirt7 served as a predictor of adverse outcome in breast cancer.
Int J Oncol
An asymmetrically dimethylarginated nuclear 90 kDa protein (p90aDMA) induced by interleukin (IL)-2, IL-4 or IL-6 in the tumor microenvironment is selectively degraded by autophagy.
Sirtuin 7 promotes non‑small cell lung cancer progression by facilitating G1/S phase and epithelial‑mesenchymal transition and activating AKT and ERK1/2 signaling.