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SIRT7 Polyclonal antibody

SIRT7 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12994-1-AP

Synonyms

SIR2 like protein 7, SIR2L7, SIRT7



Tested Applications

Positive WB detected inmouse liver tissue, human kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inrat liver tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12994-1-AP targets SIRT7 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRT7 fusion protein Ag3656
Full Name sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
Calculated molecular weight 400 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC017305
Gene symbol SIRT7
Gene ID (NCBI) 51547
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

Protocols

Product Specific Protocols
WB protocol for SIRT7 antibody 12994-1-APDownload protocol
IHC protocol for SIRT7 antibody 12994-1-APDownload protocol
IP protocol for SIRT7 antibody 12994-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Oxid Med Cell Longev

SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway.

Authors - Kun-Lin Chen
humanIHC

Front Genet

Comprehensive Analysis of Expression and Prognostic Value of Sirtuins in Ovarian Cancer.

Authors - Xiaodan Sun
humanWB

Aging Cell

Lycorine hydrochloride suppresses stress-induced premature cellular senescence by stabilizing the genome of human cells.

Authors - Weina Zhang
humanWB

Int J Clin Exp Pathol

High expression of Sirt7 served as a predictor of adverse outcome in breast cancer.

Authors - Qian Geng

Int J Oncol

An asymmetrically dimethylarginated nuclear 90 kDa protein (p90aDMA) induced by interleukin (IL)-2, IL-4 or IL-6 in the tumor microenvironment is selectively degraded by autophagy.

Authors - Lei Sun
humanWB,IHC

Oncol Rep

Sirtuin 7 promotes non‑small cell lung cancer progression by facilitating G1/S phase and epithelial‑mesenchymal transition and activating AKT and ERK1/2 signaling.

Authors - Yingying Zhao
  • KD Validated