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SIRT7 Polyclonal antibody

SIRT7 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12994-1-AP

Synonyms

SIR2 like protein 7, SIR2L7, SIRT7



Tested Applications

Positive WB detected inPC-3 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

12994-1-AP targets SIRT7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRT7 fusion protein Ag3656
Full Name sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
Calculated molecular weight 400 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC017305
Gene symbol SIRT7
Gene ID (NCBI) 51547
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

Protocols

Product Specific Protocols
WB protocol for SIRT7 antibody 12994-1-APDownload protocol
IHC protocol for SIRT7 antibody 12994-1-APDownload protocol
IP protocol for SIRT7 antibody 12994-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
mouseWB

Nat Commun

Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.

Authors - Min Liu
mouseWB

Redox Biol

A new FGF1 variant protects against adriamycin-induced cardiotoxicity via modulating p53 activity.

Authors - Mengjie Xiao
mouseWB

Cell Death Dis

MicroRNA-148a deficiency promotes hepatic lipid metabolism and hepatocarcinogenesis in mice.

Authors - Li Cheng
humanWB,IF

Sci Rep

Influence of psychostimulants and opioids on epigenetic modification of class III histone deacetylase (HDAC)-sirtuins in glial cells.

Authors - Kalaiselvi Sivalingam
humanWB

J Cell Physiol

SIRT7 depletion inhibits cell proliferation, migration and increases drug sensitivity by activating p38MAPK in breast cancer cells.

Authors - Kun-Lin Chen
  • KD Validated