Tested Applications
| Positive WB detected in | PC-3 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | mouse liver tissue, mouse stomach tissue, rat stomach tissue, rat colon tissue, mouse colon tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12994-1-AP targets SIRT7 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3656 Product name: Recombinant human SIRT7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC017305 Sequence: MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT Predict reactive species |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae) |
| Calculated Molecular Weight | 400 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC017305 |
| Gene Symbol | SIRT7 |
| Gene ID (NCBI) | 51547 |
| RRID | AB_10644276 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRC8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SIRT7 antibody 12994-1-AP | Download protocol |
| IP protocol for SIRT7 antibody 12994-1-AP | Download protocol |
| WB protocol for SIRT7 antibody 12994-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SIRT7 Polyclonal antibody (Cat# 12994-1-AP) has 45 citations across journals including Nature Communications, Cell Metabolism, Science Translational Medicine, Journal of Advanced Research, Theranostics, Advanced Science, Cell Death & Disease, Redox Biology, PLoS Pathogens, and Journal of Biological Chemistry. Research fields span oncology (breast, lung, colorectal, cervical, glioma, ovarian cancer), diabetes and kidney disease, inflammation, sepsis, aging, cardiotoxicity, ferroptosis, epigenetic regulation, lipid metabolism, and vascular biology.
| Species | Application | Title |
|---|---|---|
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Nat Commun N6-methyladenosine of Spi2a attenuates inflammation and sepsis-associated myocardial dysfunction in mice | ||
Nat Commun Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling. | ||
J Adv Res SIRT7 facilitates ferroptosis resistance of melanocytes via activating the SMAD3-ATF3-GPX4 signaling pathway in vitiligo | ||
J Adv Res Sirtuin 7-mediated deacetylation of hypoxia-inducible factor 1-alpha facilitates glycolytic reprogramming and inflammation of keratinocytes in psoriasis. | ||
Redox Biol A new FGF1 variant protects against adriamycin-induced cardiotoxicity via modulating p53 activity. |
