|Positive WB detected in||PC-3 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue|
|Positive IP detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
12994-1-AP targets SIRT7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SIRT7 fusion protein Ag3656|
|Full Name||sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)|
|Calculated molecular weight||400 aa, 45 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||BC017305|
|Gene ID (NCBI)||51547|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.
Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.
Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.
A new FGF1 variant protects against adriamycin-induced cardiotoxicity via modulating p53 activity.
Cell Death Dis
MicroRNA-148a deficiency promotes hepatic lipid metabolism and hepatocarcinogenesis in mice.
Influence of psychostimulants and opioids on epigenetic modification of class III histone deacetylase (HDAC)-sirtuins in glial cells.
J Cell Physiol
SIRT7 depletion inhibits cell proliferation, migration and increases drug sensitivity by activating p38MAPK in breast cancer cells.