SIX1 Polyclonal antibody

• 

  WB analysis of A2780 using 10709-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10709-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10709-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10709-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10709-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10709-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10709-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10709-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10709-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10709-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10709-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10709-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10709-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10709-1-AP

  IP result of anti-SIX1 (IP:10709-1-AP, 3ug; Detection:10709-1-AP 1:1000) with HEK-293 cells lysate 2560ug.

• 

  IF Staining of epithelial tumor cells using 10709-1-AP

  IF result of anti-SIX1 (1:50) in p53-/- mammary epithelial tumor cells. Blue: hoechst nuclear stain, Red: SIX1 stain the mesenchymal cell which have undergone EMT.

• Featured Product
• KD/KO Validated

SIX1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 10709-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

TIP39, SIX homeobox 1, Sine oculis homeobox homolog 1, Homeobox protein SIX1, DFNA23

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Tested Applications

• Positive WB detected in: A2780 cells, HEK-293 cells, HepG2 cells, L02 cells, PC-3 cells, SKOV-3 cells
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: epithelial tumor cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 23 publications below
• IHC: See 13 publications below
• IF: See 9 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

10709-1-AP targets SIX1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1044

  Product name: Recombinant human SIX1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-284 aa of BC008874

  Sequence: MSMLPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYVEAEKLCGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDSLLGPLTSSLVDLGS

• Full Name: SIX homeobox 1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC008874
• Gene Symbol: SIX1
• Gene ID (NCBI): 6495
• RRID: AB_2189077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15475
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li ; KO Validated
human,mouse	WB,IHC	Adv Sci (Weinh); Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells; Authors - Ling Li
mouse	WB	Nat Commun; Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology.; Authors - Nan Zhang
mouse		PLoS Biol; Dynamic enhancers control skeletal muscle identity and reprogramming.; Authors - Krithika Ramachandran
human	WB,IHC,RIP	Int J Biol Sci; Novel LncRNA LINC02936 Suppresses Ferroptosis and Promotes Tumor Progression by Interacting with SIX1/CP Axis in Endometrial Cancer; Authors - Zihui Zhang ; KD Validated
human	WB	Theranostics; O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma Proliferation.; Authors - Yi Chu ; KD Validated