|Positive IP detected in||HEK-293 cells|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
11562-1-AP targets SIX2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Alligator, canine, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SIX2 fusion protein Ag2124|
|Full Name||SIX homeobox 2|
|Calculated molecular weight||291 aa, 32 kDa|
|Observed molecular weight||32-37 kDa|
|GenBank accession number||BC024033|
|Gene ID (NCBI)||10736|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.
Lin28 and let-7 regulate the timing of cessation of murine nephrogenesis.
Disabling of nephrogenesis in porcine embryos via CRISPR/Cas9-mediated SIX1 and SIX4 gene targeting.
Nephron progenitor commitment is a stochastic process influenced by cell migration.
Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.
Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats.
Am J Physiol Renal Physiol
Loss of miR-17~92 results in dysregulation of Cftr in nephron progenitors.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Nicolas (Verified Customer) (09-17-2020)
I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)