SIX2 Polyclonal antibody

SIX2 Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

203

Reviews (1)

Cat no : 11562-1-AP

Synonyms

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

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Validation Data Gallery

• 

  WB analysis using 11562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11562-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11562-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11562-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11562-1-AP

  IP Result of anti-SIX2 (IP:11562-1-AP, 3ug; Detection:11562-1-AP 1:500) with HEK-293 cells lysate 2700ug.

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Tested Applications

• Positive IP detected in: HEK-293 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 24 publications below
• IHC: See 33 publications below
• IF: See 166 publications below
• IP: See 2 publications below
• ChIP: See 4 publications below

Product Information

11562-1-AP targets SIX2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Alligator, canine, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIX2 fusion protein Ag2124
• Full Name: SIX homeobox 2
• Calculated molecular weight: 291 aa, 32 kDa
• Observed molecular weight: 32-37 kDa
• GenBank accession number: BC024033
• Gene symbol: SIX2
• Gene ID (NCBI): 10736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599]. This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.

Publications

Species	Application	Title
mouse	WB,IHC,IF	Nat Commun; Lin28 and let-7 regulate the timing of cessation of murine nephrogenesis.; Authors - Alena V Yermalovich
pig	IHC	Xenotransplantation; Disabling of nephrogenesis in porcine embryos via CRISPR/Cas9-mediated SIX1 and SIX4 gene targeting.; Authors - Junzheng Wang
mouse	IF	Elife; Nephron progenitor commitment is a stochastic process influenced by cell migration.; Authors - Kynan T Lawlor
mouse	IF	Sci Rep; Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation.; Authors - Yoichi Murakami
rat	IF	Nat Commun; Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats.; Authors - Teppei Goto
mouse	IF	Am J Physiol Renal Physiol; Loss of miR-17~92 results in dysregulation of Cftr in nephron progenitors.; Authors - Yu Leng Phua

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nicolas (Verified Customer) (09-17-2020)

I performed an antigen retrieval (boil in citrate buffer 10mM, pH6 during 12 minutes)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: E18.5 kidney

SIX2 Antibody Immunofluorescence validation (1/200 dilution) in E18.5 kidney (Cat no:11562-1-AP)

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