|Positive WB detected in||HEK-293 cells, COLO 320 cells, HepG2 cells, human kidney tissue, Jurkat cells|
|Positive IP detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
11462-1-AP targets SKIV2L in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||SKIV2L fusion protein Ag1977|
|Full Name||superkiller viralicidic activity 2-like (S. cerevisiae)|
|Calculated molecular weight||1246 aa, 138 kDa|
|Observed molecular weight||138 kDa|
|GenBank accession number||BC015758|
|Gene ID (NCBI)||6499|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].
Biochem Biophys Res Commun
IL-1β/ATF3-mediated induction of Ski2 expression enhances hepatitis B virus x mRNA degradation.
Invest Ophthalmol Vis Sci
A genetic variant in the SKIV2L gene is significantly associated with age-related macular degeneration in a Han Chinese population.
J Biol Chem
RNA exosome complex regulates stability of Hepatitis B Virus's X-mRNA transcript in a Non-Stop mediated (NSD) RNA quality control mechanism.
J Biol Chem
The Hbs1-Dom34 Protein Complex Functions in Non-stop mRNA Decay in Mammalian Cells.
Nucleic Acids Res
A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.
Proc Natl Acad Sci U S A
The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Rachel (Verified Customer) (01-31-2019)
This antibody does produce a band at the expected size for SKIV2L, but it also lights up several lower molecular weight, non-specific bands. Additionally, it stains two different ladders that I have run as well.