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  • KD/KO Validated

SKIV2L Polyclonal antibody

SKIV2L Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11462-1-AP

Synonyms

170A, DDX13, Helicase like protein, Helicase SKI2W, HLP, SKI2, SKI2W, SKIV2, SKIV2L



Tested Applications

Positive WB detected inHEK-293 cells, COLO 320 cells, HepG2 cells, human kidney tissue, Jurkat cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

11462-1-AP targets SKIV2L in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKIV2L fusion protein Ag1977
Full Name superkiller viralicidic activity 2-like (S. cerevisiae)
Calculated molecular weight 1246 aa, 138 kDa
Observed molecular weight 138 kDa
GenBank accession numberBC015758
Gene symbol SKIV2L
Gene ID (NCBI) 6499
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

Protocols

Product Specific Protocols
WB protocol for SKIV2L antibody 11462-1-APDownload protocol
IP protocol for SKIV2L antibody 11462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

IL-1β/ATF3-mediated induction of Ski2 expression enhances hepatitis B virus x mRNA degradation.

Authors - Fumihiro Shiromoto
mouseIHC

Invest Ophthalmol Vis Sci

A genetic variant in the SKIV2L gene is significantly associated with age-related macular degeneration in a Han Chinese population.

Authors - Lu Fang F
humanWB,IF

J Biol Chem

RNA exosome complex regulates stability of Hepatitis B Virus's X-mRNA transcript in a Non-Stop mediated (NSD) RNA quality control mechanism.

Authors - Hussein H Aly
  • KD Validated
humanWB

J Biol Chem

The Hbs1-Dom34 Protein Complex Functions in Non-stop mRNA Decay in Mammalian Cells.

Authors - Saito Syuhei S
  • KD Validated
humanWB

Nucleic Acids Res

A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.

Authors - Katarzyna Kalisiak
humanWB

Proc Natl Acad Sci U S A

The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses.

Authors - Stuart Weston
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rachel (Verified Customer) (01-31-2019)

This antibody does produce a band at the expected size for SKIV2L, but it also lights up several lower molecular weight, non-specific bands. Additionally, it stains two different ladders that I have run as well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: A549 cell line