Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, COLO 320 cells, human kidney tissue, HepG2 cells, Jurkat cells
|Positive IP detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11462-1-AP targets SKIV2L in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|SKIV2L fusion protein Ag1977
|superkiller viralicidic activity 2-like (S. cerevisiae)
|Calculated molecular weight
|1246 aa, 138 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].
The SKIV2L RNA exosome limits activation of the RIG-I-like receptors.
The mammalian SKIV2L RNA exosome is essential for early B cell development.
J Clin Invest
Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.
Nucleic Acids Res
Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway.
Nucleic Acids Res
A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.
Proc Natl Acad Sci U S A
The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Rachel (Verified Customer) (01-31-2019)
This antibody does produce a band at the expected size for SKIV2L, but it also lights up several lower molecular weight, non-specific bands. Additionally, it stains two different ladders that I have run as well.