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  • KD/KO Validated

SKIV2L Polyclonal antibody

SKIV2L Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11462-1-AP

Synonyms

170A, DDX13, Helicase like protein, Helicase SKI2W, HLP, SKI2, SKI2W, SKIV2, SKIV2L



Tested Applications

Positive WB detected inHEK-293 cells, COLO 320 cells, human kidney tissue, HepG2 cells, Jurkat cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11462-1-AP targets SKIV2L in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKIV2L fusion protein Ag1977
Full Name superkiller viralicidic activity 2-like (S. cerevisiae)
Calculated molecular weight 1246 aa, 138 kDa
Observed molecular weight 138 kDa
GenBank accession numberBC015758
Gene symbol SKIV2L
Gene ID (NCBI) 6499
RRIDAB_2187472
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

Protocols

Product Specific Protocols
WB protocol for SKIV2L antibody 11462-1-APDownload protocol
IP protocol for SKIV2L antibody 11462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Immunol

The SKIV2L RNA exosome limits activation of the RIG-I-like receptors.

Authors - Sterling C Eckard
WB

Sci Immunol

The mammalian SKIV2L RNA exosome is essential for early B cell development.

Authors - Kun Yang
humanWB

J Clin Invest

Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.

Authors - Kun Yang
humanWB

Nucleic Acids Res

Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway.

Authors - Takuto Nogimori
  • KD Validated
humanWB

Nucleic Acids Res

A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.

Authors - Katarzyna Kalisiak
humanWB

Proc Natl Acad Sci U S A

The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses.

Authors - Stuart Weston
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rachel (Verified Customer) (01-31-2019)

This antibody does produce a band at the expected size for SKIV2L, but it also lights up several lower molecular weight, non-specific bands. Additionally, it stains two different ladders that I have run as well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: A549 cell line