CD229/SLAMF3 Recombinant antibody

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  WB analysis of EL-4 using 84817-4-RR

  EL-4 cells were subjected to SDS PAGE followed by western blot with 84817-4-RR (CD229/SLAMF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84817-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84817-4-RR (SLAMF3/CD229 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84817-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84817-4-RR against Mouse SLAMF3/CD229 were performed. The affinity constant is 2.04 nM.

CD229/SLAMF3 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 84817-4-RR

Clone No.242312E8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Lymphocyte antigen 9, Ly-9, Ly9, Cell surface molecule Ly-9, CD229

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Tested Applications

• Positive WB detected in: EL-4 cells, mouse thymus tissue, mouse spleen tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84817-4-RR targets CD229/SLAMF3 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lymphocyte antigen 9
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 80-90 kDa, 110 kDa
• GenBank Accession Number: NM_001277968.1
• Gene Symbol: Ly9
• Gene ID (NCBI): 17085
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q01965
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocytic activation molecule 3 (SLAMF3), also known as CD229 or Ly9, is a type I transmembrane glycoprotein that belongs to the SLAM family receptors (PMID: 10970093; 15905546). It is composed of an extracellular region, which consists of four Ig-like domains, a transmembrane region, and a cytoplasmic tail, which includes two immunoreceptor tyrosine-based signaling motifs (ITSMs) and other tyrosine residues that act as binding sites for the SH2 structural domains (PMID: 8537117; 11369645; 38626547). SLAMF3 is mainly expressed in immune cells, such as T, B, and natural killer cells, and it is involved in the fundamental activation and suppression of these immune cells, as well as the regulation of complex immune networks (PMID: 38626547).