ANT1/2 Polyclonal antibody

ANT1/2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 17796-1-AP

Synonyms

SLC25A5, ANT 2, ADP/ATP translocase 2, N-terminally processed, ADP/ATP translocase 2, ADP,ATP carrier protein 2

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Validation Data Gallery

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  WB analysis using 17796-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17796-1-AP (ANT1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat liver tissue
• Positive IHC detected in: human liver tissue, human liver cancer tissue, mouse heart tissue, mouse kidney tissue, human cervical cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 5 publications below

Product Information

17796-1-AP targets ANT1/2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANT1/2 fusion protein Ag11956
• Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
• Calculated Molecular Weight: 298 aa, 33 kDa
• Observed Molecular Weight: 30-32 kDa, 60 kDa
• GenBank Accession Number: BC056160
• Gene Symbol: SLC25A5
• Gene ID (NCBI): 292
• RRID: AB_2190358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)

Publications

Species	Application	Title
	WB	Elife; Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases.; Authors - Jiaqi Fu
mouse	WB	PLoS Genet; Novel insights into SLC25A46-related pathologies in a genetic mouse model.; Authors - Maria Eirini Terzenidou
human	IHC	Mol Cell Proteomics; Proteomics of skin proteins in psoriasis: from discovery and verification in a mouse model to confirmation in humans.; Authors - Kathleen C Lundberg
rat	WB	Exp Neurol; Physical exercise ameliorates psychiatric disorders and cognitive dysfunctions by hippocampal mitochondrial function and neuroplasticity in post-traumatic stress disorder.; Authors - Jin-Hee Seo
mouse	WB	J Proteome Res; Proteomic Identification of the SLC25A46 Interactome in Transgenic Mice Expressing SLC25A46-FLAG.; Authors - Vasiliki-Iris Perivolidi
human	WB	Biology (Basel); TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.; Authors - Yanbing Wang