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ANT1/2 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 17796-1-AP

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Con: 68 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

ANT1/2 Antibody WB mouse brain tissue 17796-1-AP

Mouse brain and rat brain tissues were subjected to SDS PAGE followed by western blot with 17796-1-AP (ANT1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ANT1/2 Antibody IHC human kidney tissue 17796-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney slide using 17796-1-AP (ANT1/2 Antibody) at dilution of 1:50

ANT1/2 Antibody IHC human kidney tissue 17796-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney slide using 17796-1-AP (ANT1/2 Antibody) at dilution of 1:50

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue
Positive IHC detected in:
human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ANT1/2 fusion protein Ag11956
Full name:
solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
Calculated molecular weight:
298aa,33 kDa
Observed molecular weight:
30-32 kDa
GenBank accession number:
BC056160
Gene ID (NCBI):
292
Gene symbol
SLC25A5
Synonyms
2F1, AAC2, ADP,ATP carrier protein 2, ADP/ATP translocase 2, ANT 2, ANT1/2, SLC25A5, T2, T3

Background

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimmer of ANT1/2. (22423112)


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