ANT1/2 Polyclonal antibody

ANT1/2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 17796-1-AP

Synonyms

2F1, AAC2, ADP,ATP carrier protein 2, ADP/ATP translocase 2, ANT 2, ANT1/2, SLC25A5, T2, T3

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Validation Data Gallery

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  WB analysis of mouse brain using 17796-1-AP

  Mouse brain and rat brain tissues were subjected to SDS PAGE followed by western blot with 17796-1-AP (ANT1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 17796-1-AP (ANT1/2 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 17796-1-AP (ANT1/2 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, human liver cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 8 publications below
• IHC: See 2 publications below

Product Information

17796-1-AP targets ANT1/2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANT1/2 fusion protein Ag11956
• Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
• Calculated molecular weight: 298 aa, 33 kDa
• Observed molecular weight: 30-32 kDa
• GenBank accession number: BC056160
• Gene symbol: SLC25A5
• Gene ID (NCBI): 292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimmer of ANT1/2. (22423112)

Publications

Species	Application	Title
mouse	WB	J Appl Physiol (1985); Combined effects of Aerobic exercise and 40Hz light flicker exposure on early cognitive impairments in Alzheimer's disease of 3xTg mice.; Authors - Sang-Seo Park
human	WB	Mol Med Rep; Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis.; Authors - Da-Wei Liu
human	IHC	Mol Imaging Biol; Adenine Nucleotide Translocase 2 as an Enzyme Related to [18F] FDG Accumulation in Various Cancers.; Authors - Chul-Hee Lee
human	WB	J Proteome Res; Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.; Authors - Foteini Violitzi
human	WB	Biology (Basel); TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.; Authors - Yanbing Wang
mouse	WB	J Proteome Res; Proteomic Identification of the SLC25A46 Interactome in Transgenic Mice Expressing SLC25A46-FLAG.; Authors - Vasiliki-Iris Perivolidi