SMAD1 Polyclonal antibody

SMAD1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 10429-1-AP

Synonyms

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, SMAD 1, SMAD family member 1, SMAD1

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Validation Data Gallery

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  WB analysis using 10429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10429-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10429-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10429-1-AP

  IP Result of anti-SMAD1 (IP:10429-1-AP, 4ug; Detection:10429-1-AP 1:500) with mouse heart tissue lysate 8000ug.

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  IF Staining of HeLa using 10429-1-AP

  Immunofluorescent analysis of HeLa cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10429-1-AP

  Immunofluorescent analysis of HepG2 cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HepG2 cells, mouse heart tissue, HeLa cells
• Positive IP detected in: mouse heart tissue
• Positive IF detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 15 publications below

Product Information

10429-1-AP targets SMAD1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMAD1 fusion protein Ag0701
• Full Name: SMAD family member 1
• Calculated molecular weight: 52 kDa
• Observed molecular weight: 52 kDa
• GenBank accession number: BC001878
• Gene symbol: SMAD1
• Gene ID (NCBI): 4086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway.; Authors - Qi Cui
mouse	WB	BMC Oral Health; ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum.; Authors - Jingyu Wang
mouse,rat	WB	Cell Death Dis; BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease.; Authors - Wei Peng
human	WB	Mol Med Rep; Abnormal expression levels of BMP15/Smad1 are associated with granulosa cell apoptosis in patients with polycystic ovary syndrome.; Authors - Xiangrong Cui
human,mouse	WB	Bioengineered; Guizhi Fuling pill attenuates liver fibrosis in vitro and in vivo via inhibiting TGF-β1/Smad2/3 and activating IFN-γ/Smad7 signaling pathways.; Authors - Zhongliang Liu
mouse	WB	BMC Pulm Med; IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation.; Authors - Zhaoxing Dong