SMAD1 Polyclonal antibody

SMAD1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 10429-1-AP

Synonyms

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, SMAD 1, SMAD family member 1, SMAD1

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Validation Data Gallery

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  WB analysis using 10429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10429-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10429-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10429-1-AP

  IP Result of anti-SMAD1 (IP:10429-1-AP, 4ug; Detection:10429-1-AP 1:500) with mouse heart tissue lysate 8000ug.

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  IF Staining of HeLa using 10429-1-AP

  Immunofluorescent analysis of HeLa cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10429-1-AP

  Immunofluorescent analysis of HepG2 cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HepG2 cells, mouse heart tissue, HeLa cells
• Positive IP detected in: mouse heart tissue
• Positive IF detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 20 publications below

Product Information

10429-1-AP targets SMAD1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMAD1 fusion protein Ag0701
• Full Name: SMAD family member 1
• Calculated molecular weight: 52 kDa
• Observed molecular weight: 52 kDa
• GenBank accession number: BC001878
• Gene symbol: SMAD1
• Gene ID (NCBI): 4086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase

Publications

Species	Application	Title
mouse	WB	Carbohydr Polym; In vitro and in silico anti-osteoporosis activities and underlying mechanisms of a fructan, ABW90-1, from Achyranthes bidentate.; Authors - Tianyu Li
mouse	WB	Cell Death Discov; TAZ promotes osteogenic differentiation of mesenchymal stem cells line C3H10T1/2, murine multi-lineage cells lines C2C12, and MEFs induced by BMP9; Authors - Huakun Huang
mouse	WB	Front Immunol; Silencing SOCS3 Markedly Deteriorates Spondyloarthritis in Mice Induced by Minicircle DNA Expressing IL23.; Authors - Yuhai Chen
mouse	WB	Front Mol Neurosci; Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.; Authors - Tianda Chen
mouse	WB	Ecotoxicol Environ Saf; Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.; Authors - Di Wang
rat	WB	J Cell Physiol; Aspirin promotes tenogenic differentiation of tendon stem cells and facilitates tendinopathy healing through regulating the GDF7/Smad1/5 signaling pathway.; Authors - Yunjiao Wang