SMAD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMAD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 10429-1-AP

Synonyms

MADH1, JV4-1, JV41, hSMAD1, BSP-1

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Validation Data Gallery

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  WB analysis using 10429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10429-1-AP

  WB result of SMAD1 antibody (10429-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD1 transfected HEK-293 cells.

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  WB analysis of HepG2 using 10429-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10429-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10429-1-AP

  IP result of anti-SMAD1 (IP:10429-1-AP, 4ug; Detection:10429-1-AP 1:500) with mouse heart tissue lysate 8000ug.

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  IHC staining of human colon using 10429-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10429-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 10429-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 10429-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10429-1-AP

  Immunofluorescent analysis of HeLa cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10429-1-AP

  Immunofluorescent analysis of HepG2 cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HEK-293 cells, HepG2 cells, mouse heart tissue, HeLa cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human colon tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 27 publications below

Product Information

10429-1-AP targets SMAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMAD1 fusion protein Ag0701
• Full Name: SMAD family member 1
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC001878
• Gene Symbol: SMAD1
• Gene ID (NCBI): 4086
• RRID: AB_2247334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase

Publications

Species	Application	Title
mouse	WB	Carbohydr Polym; In vitro and in silico anti-osteoporosis activities and underlying mechanisms of a fructan, ABW90-1, from Achyranthes bidentate.; Authors - Tianyu Li
mouse	WB	Cell Death Discov; TAZ promotes osteogenic differentiation of mesenchymal stem cells line C3H10T1/2, murine multi-lineage cells lines C2C12, and MEFs induced by BMP9; Authors - Huakun Huang
mouse	WB	Front Immunol; Silencing SOCS3 Markedly Deteriorates Spondyloarthritis in Mice Induced by Minicircle DNA Expressing IL23.; Authors - Yuhai Chen
mouse	WB	Front Mol Neurosci; Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.; Authors - Tianda Chen
human	WB	IUBMB Life; LncRNA RP11-301G19.1 is required for the maintenance of vascular smooth muscle cell contractile phenotype via sponging miR-17-5P/ATOH8 axis; Authors - Shuning Hao
mouse	WB	Ecotoxicol Environ Saf; Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.; Authors - Di Wang