SMAD3 Monoclonal antibody

SMAD3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

96

CloneNo.

1F10B2

Reviews (1)

Cat no : 66516-1-Ig

Synonyms

JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3

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Validation Data Gallery

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  WB analysis using 66516-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66516-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 66516-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 66516-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-1080 cells, NIH/3T3 cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells
• Positive IHC detected in: human stomach cancer tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 90 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below

Product Information

66516-1-Ig targets SMAD3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SMAD family member 3
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: NM_005902
• Gene Symbol: SMAD3
• Gene ID (NCBI): 4088
• RRID: AB_2881879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu
human	WB	Cancer Lett; Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma; Authors - Qianyu Shi
human,mouse	WB	Cell Chem Biol; Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction; Authors - Wanqi Yang
mouse	WB,IF	Cell Mol Life Sci; Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion; Authors - Shiju Ye
human	CoIP	Diabetes; Overexpressed Poldip2 incurs retinal fibrosis via the TGFβ1/SMAD3 signaling pathway in diabetic retinopathy; Authors - Zhiyu Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Juliette (Verified Customer) (11-25-2024)

Good

• Applications: Immunofluorescence
• Cell Tissue Type: Human