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SMAD3 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66516-1-Ig |CloneNo.: 1F10B2

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, ELISA
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SMAD3 Antibody WB Jurkat cells 66516-1-Ig

Various cells were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

Species specificity:
Human, mouse
Positive WB detected in:
Jurkat cells, HeLa cells
Recommended dilution:
WB : 1:1000-1:6000

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
SMAD family member 3
Calculated molecular weight:
48 kDa
Observed molecular weight:
55-60 kDa
GenBank accession number:
NM_005902
Gene ID (NCBI):
4088
Gene symbol
SMAD3
Synonyms
hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3

Background

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.


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