SMAD3 Monoclonal antibody
SMAD3 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse and More (1)
Applications
WB,ELISA
Conjugate
Unconjugated
CloneNo.
1F10B2
Cat no : 66516-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-1080 cells, NIH/3T3 cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 68 publications below |
IHC | See 1 publications below |
IF | See 5 publications below |
CoIP | See 1 publications below |
Product Information
66516-1-Ig targets SMAD3 in WB,ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | SMAD family member 3 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 55-60 kDa |
GenBank Accession Number | NM_005902 |
Gene Symbol | SMAD3 |
Gene ID (NCBI) | 4088 |
RRID | AB_2881879 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.
Protocols
Product Specific Protocols | |
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WB protocol for SMAD3 antibody 66516-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK | ||
Drug Des Devel Ther Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling | ||
Cell Chem Biol Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction | ||
Cell Mol Life Sci Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion | ||
Oxid Med Cell Longev Smad7 Is Highly Expressed in Human Degenerative Discs and Participates in IL-1β-Induced Apoptosis of Rat AF Cells via the Mitochondria Pathway. | ||
Mol Ther Nucleic Acids Genetic or siRNA inhibition of MBD2 attenuates the UUO- and I/R-induced renal fibrosis via downregulation of EGR1. |