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SMAD5 Polyclonal antibody

SMAD5 Polyclonal Antibody for FC, IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12167-1-AP


Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12167-1-AP targets SMAD5 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAD5 fusion protein Ag2809
Full Name SMAD family member 5
Calculated molecular weight 465 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC009682
Gene symbol SMAD5
Gene ID (NCBI) 4090
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.


Product Specific Protocols
WB protocol for SMAD5 antibody 12167-1-APDownload protocol
IF protocol for SMAD5 antibody 12167-1-APDownload protocol
IP protocol for SMAD5 antibody 12167-1-APDownload protocol
FC protocol for SMAD5 antibody 12167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Biophys Res Commun

SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.

Authors - Jinliang Chen

PLoS Biol

BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.

Authors - Christian Hiepen

Signal Transduct Target Ther

Breast cancer-derived exosomes transmit lncRNA SNHG16 to induce CD73+γδ1 Treg cells.

Authors - Chao Ni

JCI Insight

Splicing factor SRSF6 mediates pleural fibrosis.

Authors - Li-Mei Liang

Genes Dis

Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells.

Authors - Xiaogen Ma

Cell Death Discov

Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling

Authors - Ping Yin