Validation Data Gallery
|Positive WB detected in||K-562 cells|
|Positive IP detected in||K-562 cells|
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12167-1-AP targets SMAD5 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SMAD5 fusion protein Ag2809|
|Full Name||SMAD family member 5|
|Calculated molecular weight||465 aa, 52 kDa|
|Observed molecular weight||52 kDa|
|GenBank accession number||BC009682|
|Gene ID (NCBI)||4090|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.
|Product Specific Protocols|
|WB protocol for SMAD5 antibody 12167-1-AP||Download protocol|
|IF protocol for SMAD5 antibody 12167-1-AP||Download protocol|
|IP protocol for SMAD5 antibody 12167-1-AP||Download protocol|
|FC protocol for SMAD5 antibody 12167-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochem Biophys Res Commun
SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.
BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.
Signal Transduct Target Ther
Breast cancer-derived exosomes transmit lncRNA SNHG16 to induce CD73+γδ1 Treg cells.
Splicing factor SRSF6 mediates pleural fibrosis.
Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells.
Cell Death Discov
Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling