SMAD5 Polyclonal antibody

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  WB analysis of K-562 using 12167-1-AP

  WB result of SMAD5 antibody (12167-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD5 transfected K-562 cells.

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  WB analysis using 12167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12167-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 12167-1-AP

  IP result of anti-SMAD5 (IP:12167-1-AP, 4ug; Detection:12167-1-AP 1:5000) with HeLa cells lysate 1760 ug.

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  IF Staining of MCF-7 using 12167-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SMAD5 antibody 12167-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 12167-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMAD5 antibody (12167-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of HeLa using 12167-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMAD5 antibody (12167-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

SMAD5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 12167-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

MADH5, MAD homolog 5, JV5-1, JV5 1, hSmad5

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, NIH/3T3 cells, PC-12 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Product Information

12167-1-AP targets SMAD5 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMAD5 fusion protein Ag2809
• Full Name: SMAD family member 5
• Calculated Molecular Weight: 465 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC009682
• Gene Symbol: SMAD5
• Gene ID (NCBI): 4090
• RRID: AB_2286502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99717
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.

Publications

Species	Application	Title
		PLoS Biol; BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.; Authors - Christian Hiepen
human	WB	IUBMB Life; LncRNA RP11-301G19.1 is required for the maintenance of vascular smooth muscle cell contractile phenotype via sponging miR-17-5P/ATOH8 axis; Authors - Shuning Hao
human	FC	Sci Rep; IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation.; Authors - Gina Dörpholz
rat	WB	Int J Biochem Cell Biol; The Role of SIRT6 in the Differentiation of Vascular Smooth Muscle Cells in Response to Cyclic Strain.; Authors - Qing-Ping Yao
mouse	WB	Sci Rep; Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.; Authors - Xueni Wang
mouse	WB	PLoS One; Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.; Authors - Zhang Xing-Yi XY