SMAD5 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMAD5 Polyclonal Antibody for FC, IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 12167-1-AP

Synonyms

Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

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Validation Data Gallery

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  WB analysis of K-562 using 12167-1-AP

  WB result of SMAD5 antibody (12167-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD5 transfected K-562 cells.

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  WB analysis of K-562 using 12167-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of K-562 using 12167-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of K-562 using 12167-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12167-1-AP

  IP Result of anti-SMAD5 (IP:12167-1-AP, 4ug; Detection:12167-1-AP 1:1000) with K-562 cells lysate 3200ug.

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  IF Staining of MCF-7 using 12167-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SMAD5 antibody 12167-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of MCF-7 using 12167-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug SMAD5 antibody (12167-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: K-562 cells
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• FC: See 1 publications below

Product Information

12167-1-AP targets SMAD5 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMAD5 fusion protein Ag2809
• Full Name: SMAD family member 5
• Calculated molecular weight: 465 aa, 52 kDa
• Observed molecular weight: 52 kDa
• GenBank accession number: BC009682
• Gene symbol: SMAD5
• Gene ID (NCBI): 4090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.

Publications

Species	Application	Title
human	FC	Sci Rep; IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation.; Authors - Gina Dörpholz
rat	WB	Int J Biochem Cell Biol; The Role of SIRT6 in the Differentiation of Vascular Smooth Muscle Cells in Response to Cyclic Strain.; Authors - Qing-Ping Yao
mouse	WB	Sci Rep; Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.; Authors - Xueni Wang
mouse	WB	PLoS One; Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.; Authors - Zhang Xing-Yi XY
human	WB	Life Sci; Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro.; Authors - Rui Mu
rat	WB	Sci Rep; Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells.; Authors - Yan Zeng