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SMAD5 Polyclonal antibody

SMAD5 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12167-1-AP


Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, NIH/3T3 cells, PC-12 cells
Positive IP detected inK-562 cells
Positive IF detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12167-1-AP targets SMAD5 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAD5 fusion protein Ag2809
Full Name SMAD family member 5
Calculated Molecular Weight 465 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC009682
Gene Symbol SMAD5
Gene ID (NCBI) 4090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.


Product Specific Protocols
WB protocol for SMAD5 antibody 12167-1-APDownload protocol
IF protocol for SMAD5 antibody 12167-1-APDownload protocol
IP protocol for SMAD5 antibody 12167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



PLoS Biol

BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.

Authors - Christian Hiepen

Sci Rep

IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation.

Authors - Gina Dörpholz

Int J Biochem Cell Biol

The Role of SIRT6 in the Differentiation of Vascular Smooth Muscle Cells in Response to Cyclic Strain.

Authors - Qing-Ping Yao

Sci Rep

Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.

Authors - Xueni Wang

PLoS One

Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.

Authors - Zhang Xing-Yi XY

Biochem Biophys Res Commun

SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.

Authors - Jinliang Chen