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- KD/KO Validated
SMAD5 Polyclonal antibody
SMAD5 Polyclonal Antibody for FC, IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 12167-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells |
Positive IP detected in | K-562 cells |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 15 publications below |
FC | See 1 publications below |
Product Information
12167-1-AP targets SMAD5 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SMAD5 fusion protein Ag2809 |
Full Name | SMAD family member 5 |
Calculated molecular weight | 465 aa, 52 kDa |
Observed molecular weight | 52 kDa |
GenBank accession number | BC009682 |
Gene symbol | SMAD5 |
Gene ID (NCBI) | 4090 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.
Protocols
Product Specific Protocols | |
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WB protocol for SMAD5 antibody 12167-1-AP | Download protocol |
IF protocol for SMAD5 antibody 12167-1-AP | Download protocol |
IP protocol for SMAD5 antibody 12167-1-AP | Download protocol |
FC protocol for SMAD5 antibody 12167-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Rep IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation. | ||
Int J Biochem Cell Biol The Role of SIRT6 in the Differentiation of Vascular Smooth Muscle Cells in Response to Cyclic Strain. | ||
Sci Rep Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis. | ||
PLoS One Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice. | ||
Life Sci Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro. | ||
Sci Rep Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells. |