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SMAD5 Polyclonal antibody

SMAD5 Polyclonal Antibody for FC, IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12167-1-AP

Synonyms

Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5



Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12167-1-AP targets SMAD5 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAD5 fusion protein Ag2809
Full Name SMAD family member 5
Calculated molecular weight 465 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC009682
Gene symbol SMAD5
Gene ID (NCBI) 4090
RRIDAB_2286502
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.

Protocols

Product Specific Protocols
WB protocol for SMAD5 antibody 12167-1-APDownload protocol
IF protocol for SMAD5 antibody 12167-1-APDownload protocol
IP protocol for SMAD5 antibody 12167-1-APDownload protocol
FC protocol for SMAD5 antibody 12167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

PLoS Biol

BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.

Authors - Christian Hiepen
humanFC

Sci Rep

IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation.

Authors - Gina Dörpholz
ratWB

Int J Biochem Cell Biol

The Role of SIRT6 in the Differentiation of Vascular Smooth Muscle Cells in Response to Cyclic Strain.

Authors - Qing-Ping Yao
mouseWB

Sci Rep

Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.

Authors - Xueni Wang
mouseWB

PLoS One

Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.

Authors - Zhang Xing-Yi XY
humanWB

Biochem Biophys Res Commun

SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.

Authors - Jinliang Chen