|Positive WB detected in
|Positive IP detected in
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IF : 1:10-1:100
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12167-1-AP targets SMAD5 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|SMAD5 fusion protein Ag2809
|SMAD family member 5
|Calculated molecular weight
|465 aa, 52 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.
|Product Specific Protocols
|WB protocol for SMAD5 antibody 12167-1-AP
|IF protocol for SMAD5 antibody 12167-1-AP
|IP protocol for SMAD5 antibody 12167-1-AP
|FC protocol for SMAD5 antibody 12167-1-AP
|Click here to view our Standard Protocols
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