|Positive WB detected in||pig brain tissue, pig kidney tissue, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse skeletal muscle tissue|
|Positive IHC detected in||mouse cerebellum tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66478-1-Ig targets SMAD7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||SMAD7 fusion protein Ag13688|
|Full Name||SMAD family member 7|
|Calculated molecular weight||426 aa, 46 kDa|
|Observed molecular weight||46 kDa|
|GenBank accession number||BC074819|
|Gene ID (NCBI)||4092|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Aging (Albany NY)
LINC00968 can inhibit the progression of lung adenocarcinoma through the miR-21-5p/SMAD7 signal axis.
Acta Pharmacol Sin
The multifunctional adaptor protein HIP-55 couples Smad7 to accelerate TGF-β type I receptor degradation.
Biochem Biophys Res Commun
Emodin ameliorates tubulointerstitial fibrosis in obstructed kidneys by inhibiting EZH2.
Exp Cell Res
Exosomes derived from thymic stromal lymphopoietin-treated dendritic cells regulate T helper 17/regulatory T cell differentiation via miR-21/Smad7 axis.
Wu-Mei-Wan ameliorates chronic colitis-associated intestinal fibrosis through inhibiting fibroblast activation.
Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.