SMARCA4 / BRG1 antibody (mAb) (Clone 3G4)
Host / Isotype
Rat / IgG2b
Reactivity
Human, Mouse
Applications
ICC, IF, WB
CloneNo.
3G4
Cat No : 39807,39808 39807
Synonyms
Validation Data Gallery
Product Information
| Tested Applications | ICC, IF, WB |
| Tested Reactivity | Human, Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This SMARCA4 / BRG1 antibody was raised against a recombinant protein corresponding to amino acids 214-279 of mouse BRG1. |
| Full Name | SMARCA4 / BRG1 antibody (mAb) (Clone 3G4) |
| Synonyms | SMARCA4 / BRG1, SMARCA4, BRG, brg, BRG-1, BRG1, Brg1, brg-1, pAb, monoclonal, Brahma-related gene-1, SNF2 beta, antibody, antibodies, clone 3G4, stem cell, stem cells, trithorax, trxg, trx, sample |
| Molecular weight | 180 kDa |
| GenBank accession number | NP_035547 |
| RRID | AB_2614949 |
| Purification Method | Protein G Chromatography |
| Buffer | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), also known as BRG1, is the central catalytic ATPase subunit of numerous chromatin-remodeling complexes, including SWI/SNF. These complexes are able to use energy from ATP hydrolysis to physically disrupt chromatin architecture of target promoters and facilitate the binding of transcription factors to nucleosomal DNA. SMARCA4 is a co-regulator of transcription and has been implicated in the activation and repression of gene expression through the modulation of chromatin in various tissues and physiological conditions. This protein plays a major role in many cellular processes such as DNA replication, repair and recombination. Mammalian SMARCA4 is usually associated with approximately 10-12 BAF subunits or other proteins involved in regulation of gene expression. SMARCA4 is part of the stem cell-specific BAF complex, esBAF, and is involved in regulating stem cell pluripotency.