• Featured Product
  • KD/KO Validated

SMCR7L/MID51 Antibody

SMCR7L/MID51 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 20164-1-AP

Synonyms

HSU79252, MID51, MIEF1, SMCR7L



Tested Applications

Positive WB detected inHT-1080 cells, mouse heart tissue, mouse testis tissue, NIH/3T3 cells, rat liver tissue, rat testis tissue
Positive IP detected inRAW 264.7 cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The 20164-1-AP immunogen is Fusion Protein SMCR7L/MID51 protein expressed in E. coli.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMCR7L/MID51 fusion protein Ag13775
Full Name Smith-Magenis syndrome chromosome region, candidate 7-like
Calculated molecular weight 463aa,51 kDa
Observed molecular weight 48-51 kDa
GenBank accession number BC002587
Gene symbol SMCR7L
Gene ID (NCBI) 54471
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.

Protocols

Product Specific Protocols
WB protocol for SMCR7L/MID51 antibody 20164-1-APDownload protocol
IF protocol for SMCR7L/MID51 antibody 20164-1-APDownload protocol
IP protocol for SMCR7L/MID51 antibody 20164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder.

Authors - Chih-Wei Chen
ratWB

Evid Based Complement Alternat Med

Huangqi-Danshen Decoction Ameliorates Adenine-Induced Chronic Kidney Disease by Modulating Mitochondrial Dynamics.

Authors - Xinhui Liu
ratWB

Front Cardiovasc Med

Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities.

Authors - Ping Yu Xiong
humanWB

Nat Commun

ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects.

Authors - Yuanyuan Zhao
humanWB

Nat Commun

STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism.

Authors - Hongxu Xian
  • KD Validated
humanWB

Cell Death Dis

Apogossypol-mediated reorganisation of the endoplasmic reticulum antagonises mitochondrial fission and apoptosis.

Authors - Govindaraju Yedida