• Featured Product
  • KD/KO Validated

SMCR7L/MID51 Polyclonal antibody

SMCR7L/MID51 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20164-1-AP

Synonyms

HSU79252, MID51, MIEF1, SMCR7L, SMCR7L/MID51



Tested Applications

Positive WB detected inA549 cells, mouse testis tissue, NIH/3T3 cells, RAW264.7, HEK-293T cells, HT-1080 cells
Positive IP detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20164-1-AP targets SMCR7L/MID51 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMCR7L/MID51 fusion protein Ag13775
Full Name Smith-Magenis syndrome chromosome region, candidate 7-like
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight 48-51 kDa
GenBank accession numberBC002587
Gene symbol SMCR7L
Gene ID (NCBI) 54471
RRIDAB_10639522
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.

Protocols

Product Specific Protocols
WB protocol for SMCR7L/MID51 antibody 20164-1-APDownload protocol
IP protocol for SMCR7L/MID51 antibody 20164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
monkeyWB

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
  • KO Validated
humanWB

Autophagy

Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.

Authors - Hongxu Xian
  • KD Validated
mouseWB

Sci Adv

Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.

Authors - Xinwen Zhang
humanWB

Nat Commun

Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease.

Authors - Di Hu