SMCR7L/MID51 Polyclonal antibody

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  WB analysis using 20164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 20164-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 20164-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20164-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20164-1-AP (SMCR7L/MID51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of RAW 264.7 using 20164-1-AP

  IP result of anti-SMCR7L/MID51 (IP:20164-1-AP, 3ug; Detection:20164-1-AP 1:1000) with RAW 264.7 cells lysate 2400ug.

• Featured Product
• KD/KO Validated

SMCR7L/MID51 Polyclonal Antibody for WB, IP, ELISA

Cat No. 20164-1-AP

Publications(73)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

HSU79252, MID51, MIEF1, SMCR7L, SMCR7L/MID51

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Tested Applications

• Positive WB detected in: A549 cells, mouse testis tissue, NIH/3T3 cells, RAW264.7, HEK-293T cells, HT-1080 cells
• Positive IP detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 72 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• FC: See 1 publications below

Product Information

20164-1-AP targets SMCR7L/MID51 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMCR7L/MID51 fusion protein Ag13775
• Full Name: Smith-Magenis syndrome chromosome region, candidate 7-like
• Calculated Molecular Weight: 463 aa, 51 kDa
• Observed Molecular Weight: 48-51 kDa
• GenBank Accession Number: BC002587
• Gene Symbol: SMCR7L
• Gene ID (NCBI): 54471
• RRID: AB_10639522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQG6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.

Publications

Species	Application	Title
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KO Validated
human	WB,FC	Mol Cell; Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology; Authors - Gayathri Muthukumar
human	WB	Autophagy; Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.; Authors - Hongxu Xian ; KD Validated
mouse	WB	Sci Adv; Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.; Authors - Xinwen Zhang