SNAI2 Polyclonal antibody

SNAI2 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

103

Cat no : 12129-1-AP

Synonyms

Protein snail homolog 2, SLUG, SLUGH, SLUGH1, SNAI2, snail homolog 2 (Drosophila), WS2D, Zinc finger protein SNAI2

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Validation Data Gallery

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  WB analysis using 12129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12129-1-AP (SNAI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 12129-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human SNAI2 (12129-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse spleen tissue
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 92 publications below
• IHC: See 13 publications below
• IF: See 5 publications below

Product Information

12129-1-AP targets SNAI2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAI2 fusion protein Ag2773
• Full Name: snail homolog 2 (Drosophila)
• Calculated molecular weight: 268 aa, 30 kDa
• Observed molecular weight: 30 kDa
• GenBank accession number: BC015895
• Gene symbol: SNAI2
• Gene ID (NCBI): 6591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.

Publications

Species	Application	Title
human	WB	Mol Ther; IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.; Authors - Hailong Ruan
mouse	IF, WB	Theranostics; Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.; Authors - Shuyu Guo
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou
human	WB	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li
mouse	WB,IHC	Oncogene; Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.; Authors - Yongyun Li
human	IHC	Oncogene; SP1-activated long noncoding RNA lncRNA GCMA functions as a competing endogenous RNA to promote tumor metastasis by sponging miR-124 and miR-34a in gastric cancer.; Authors - Yaru Tian

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