SNAI2 Polyclonal antibody

SNAI2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

88

Cat no : 12129-1-AP

Synonyms

Protein snail homolog 2, SLUG, SLUGH, SLUGH1, SNAI2, snail homolog 2 (Drosophila), WS2D, Zinc finger protein SNAI2

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Validation Data Gallery

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  WB analysis using 12129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12129-1-AP (SNAI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12129-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12129-1-AP (SNAI2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12129-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12129-1-AP (SNAI2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 12129-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SNAI2 antibody (12129-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 12129-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human SNAI2 (12129-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse spleen tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 77 publications below
• IHC: See 13 publications below
• IF: See 5 publications below

Product Information

12129-1-AP targets SNAI2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAI2 fusion protein Ag2773
• Full Name: snail homolog 2 (Drosophila)
• Calculated molecular weight: 268 aa, 30 kDa
• Observed molecular weight: 30 kDa
• GenBank accession number: BC015895
• Gene symbol: SNAI2
• Gene ID (NCBI): 6591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p.; Authors - Wei Wu
human	WB	Cancer Manag Res; Lipase member H is a downstream molecular target of hypoxia inducible factor-1α and promotes papillary thyroid carcinoma cell migration in BCPAP and KTC-1 cell lines.; Authors - Yuefeng Li
human	WB	Theranostics; Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme.; Authors - Yaodong Chen
human	WB	J Exp Clin Cancer Res; Nuciferine inhibits the progression of glioblastoma by suppressing the SOX2-AKT/STAT3-Slug signaling pathway.; Authors - Zizhuo Li
human	WB,IF	Cell Death Dis; M2 macrophages promote NSCLC metastasis by upregulating CRYAB.; Authors - Zhe Guo
human	WB	Onco Targets Ther; Nr5a2 promotes tumor growth and metastasis of gastric cancer AGS cells by Wnt/beta-catenin signaling.; Authors - Lei Liu

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