SNAI2/SLUG Polyclonal antibody

SNAI2/SLUG Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

127

Reviews (1)

Cat no : 12129-1-AP

Synonyms

Protein snail homolog 2, SLUG, SLUGH, SLUGH1, SNAI2, snail homolog 2 (Drosophila), WS2D, Zinc finger protein SNAI2

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Validation Data Gallery

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  WB analysis using 12129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12129-1-AP (SNAI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12129-1-AP (SNAI2/SLUG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of MCF-7 using 12129-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human SNAI2 (12129-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse brain tissue, rat heart tissue, mouse spleen tissue
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 116 publications below
• IHC: See 14 publications below
• IF: See 5 publications below

Product Information

12129-1-AP targets SNAI2/SLUG in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAI2/SLUG fusion protein Ag2773
• Full Name: snail homolog 2 (Drosophila)
• Calculated molecular weight: 268 aa, 30 kDa
• Observed molecular weight: 30 kDa
• GenBank accession number: BC015895
• Gene symbol: SNAI2
• Gene ID (NCBI): 6591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.

Publications

Species	Application	Title
human	WB	Cell Death Dis; UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer; Authors - Xiaoling Hong
human	WB	Mol Ther; IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.; Authors - Hailong Ruan
mouse	IF, WB	Theranostics; Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.; Authors - Shuyu Guo
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou
mouse	WB	Int J Biol Macromol; Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression; Authors - Yu-Wei Yen
human	WB	Oncogene; N4-acetylcytidine modification of lncRNA CTC-490G23.2 promotes cancer metastasis through interacting with PTBP1 to increase CD44 alternative splicing; Authors - Xiao-Mei Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:500

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Glioblastoma cells

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