SNAI2/SLUG Polyclonal antibody

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  WB analysis using 12129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12129-1-AP (SNAI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12129-1-AP (SNAI2/SLUG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 12129-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12129-1-AP (SNAI2/SLUG antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MCF-7 using 12129-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human SNAI2 (12129-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SNAI2/SLUG Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 12129-1-AP

Publications(189)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), CoIP, ChIP, ELISA

Synonyms

SNAI2, WS2D, SLUGH1, SLUGH, SLUG

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse brain tissue, rat heart tissue, mouse spleen tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 170 publications below
• IHC: See 21 publications below
• IF: See 10 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

12129-1-AP targets SNAI2/SLUG in WB, IHC, IF, FC (Intra), CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAI2/SLUG fusion protein Ag2773
• Full Name: snail homolog 2 (Drosophila)
• Calculated Molecular Weight: 268 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC015895
• Gene Symbol: SLUG
• Gene ID (NCBI): 6591
• RRID: AB_2191889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43623
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB	J Exp Clin Cancer Res; Lnc AC016727.1/BACH1/HIF-1 α signal loop promotes the progression of non-small cell lung cancer; Authors - Li Zhang
human	WB	Cell Death Dis; UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer; Authors - Xiaoling Hong
human	WB	Mol Ther; IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.; Authors - Hailong Ruan
mouse	IF, WB	Theranostics; Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.; Authors - Shuyu Guo
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:500

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Glioblastoma cells