|Positive WB detected in||mouse brain tissue, mouse spleen tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
12129-1-AP targets SNAI2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SNAI2 fusion protein Ag2773|
|Full Name||snail homolog 2 (Drosophila)|
|Calculated molecular weight||268 aa, 30 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC015895|
|Gene ID (NCBI)||6591|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.
|Product Specific Protocols|
|WB protocol for SNAI2 antibody 12129-1-AP||Download protocol|
|IHC protocol for SNAI2 antibody 12129-1-AP||Download protocol|
|IF protocol for SNAI2 antibody 12129-1-AP||Download protocol|
|FC protocol for SNAI2 antibody 12129-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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