SNAI2 Polyclonal antibody
SNAI2 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 12129-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse spleen tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 49 publications below |
| IHC | See 6 publications below |
| IF | See 5 publications below |
Product Information
12129-1-AP targets SNAI2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SNAI2 fusion protein Ag2773 |
| Full Name | snail homolog 2 (Drosophila) |
| Calculated molecular weight | 268 aa, 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC015895 |
| Gene symbol | SNAI2 |
| Gene ID (NCBI) | 6591 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SNAI2 antibody 12129-1-AP | Download protocol |
| IHC protocol for SNAI2 antibody 12129-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p. | ||
Cancer Manag Res Lipase member H is a downstream molecular target of hypoxia inducible factor-1α and promotes papillary thyroid carcinoma cell migration in BCPAP and KTC-1 cell lines. | ||
Theranostics Oncogenic MSH6-CXCR4-TGFB1 Feedback Loop: A Novel Therapeutic Target of Photothermal Therapy in Glioblastoma Multiforme. | ||
J Exp Clin Cancer Res Nuciferine inhibits the progression of glioblastoma by suppressing the SOX2-AKT/STAT3-Slug signaling pathway. | ||
Onco Targets Ther Nr5a2 promotes tumor growth and metastasis of gastric cancer AGS cells by Wnt/beta-catenin signaling. |
