Tested Applications
| Positive WB detected in | mouse brain tissue, SH-SY5Y cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells, PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14903-1-AP targets SNAP25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6695 Product name: Recombinant human SNAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-206 aa of BC010647 Sequence: MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLDRVEEGMNHINQDMKEAEKNLKDLGKCCGLFICPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG Predict reactive species |
| Full Name | synaptosomal-associated protein, 25kDa |
| Calculated Molecular Weight | 23 kDa |
| Observed Molecular Weight | 25-27 kDa |
| GenBank Accession Number | BC010647 |
| Gene Symbol | SNAP25 |
| Gene ID (NCBI) | 6616 |
| RRID | AB_2192051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P60880 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by AX type E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SNAP25 antibody 14903-1-AP | Download protocol |
| IHC protocol for SNAP25 antibody 14903-1-AP | Download protocol |
| IP protocol for SNAP25 antibody 14903-1-AP | Download protocol |
| WB protocol for SNAP25 antibody 14903-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
SNAP25 antibody (Cat# 14903-1-AP) has accumulated 41 citations across journals including Signal Transduction and Targeted Therapy, Science Advances, Alzheimer's & Dementia, Cell Reports, eLife, Molecular Neurobiology, International Immunopharmacology, and Neuroscience. Associated research fields include neuroscience, neurodegeneration (Alzheimer's and Parkinson's disease), neuroinflammation, synaptic transmission, SNARE complex assembly and exocytosis, diabetes, pain, and spinal cord injury.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Overexpression of STX11 alleviates pulmonary fibrosis by inhibiting fibroblast activation via the PI3K/AKT/mTOR pathway | ||
Mol Neurodegener Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing. | ||
Int J Oral Sci METTL3-mediated m6A RNA methylation regulates dorsal lingual epithelium homeostasis. | ||
J Adv Res Conditional deletion of MDM2 in neurons impairs neuronal homeostasis and cognitive ability. | ||
Sci Adv Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE complex assembly and neurotransmitter release | ||
Alzheimers Dement Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer's disease and the Dp16 mouse Down syndrome model: Impact of APP gene dose |
Reviews
What customers say
Both reviewers gave this SNAP-25 antibody a perfect 5-star rating for Western Blot. One user (James Quinn) tested it on Neurogenin 2 iPSC neurons at 1:1000 dilution and reported it worked great with extreme specificity. The other (Q Chen) used it on human postmortem brain lysate at 1:5000 dilution, praising its high titer, specificity, and clean results. Overall, users find this antibody highly specific and reliable for WB applications across different sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
James (Verified Customer) (09-29-2021) | Antibody worked great and was extremely specific to SNAP-25.
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Q (Verified Customer) (03-22-2021) | An excellent SNAP25 Ab with a high titer. Very specific and clean.
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