Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, Jurkat cells, U2OS cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, A431 cells, LNCaP cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas tissue, human breast hyperplasia tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60265-1-Ig targets SND1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1200 Product name: Recombinant human SND1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 548-911 aa of BC017180 Sequence: TCLITFLLAGIECPRGARNLPGLVQEGEPFSEEATLFTKELVLQREVEVEVESMDKAGNFIGWLHIDGANLSVLLVEHALSKVHFTAERSSYYKSLLSAEEAAKQKKEKVWAHYEEQPVEEVMPVLEEKERSASYKPVFVTEITDDLHFYVQDVETGTQLEKLMENMRNDIASHPPVEGSYAPRRGEFCIAKFVDGEWYRARVEKVESPAKIHVFYIDYGNREVLPSTRLGTLSPAFSTRVLPAQATEYAFAFIQVPQDDDARTDAVDSVVRDIQNTQCLLNVEHLSAGCPHVTLQFADSKGDVGLGLVKEGLVMVEVRKEKQFQKVITEYLNAQESAKSARLNLWRYGDFRADDADEFGYSR Predict reactive species |
| Full Name | staphylococcal nuclease and tudor domain containing 1 |
| Calculated Molecular Weight | 101 kDa |
| Observed Molecular Weight | 101 kDa |
| GenBank Accession Number | BC017180 |
| Gene Symbol | SND1 |
| Gene ID (NCBI) | 27044 |
| RRID | AB_2881386 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q7KZF4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SND1 antibody 60265-1-Ig | Download protocol |
| IHC protocol for SND1 antibody 60265-1-Ig | Download protocol |
| IP protocol for SND1 antibody 60265-1-Ig | Download protocol |
| WB protocol for SND1 antibody 60265-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SND1 Monoclonal antibody (Cat# 60265-1-Ig) has 13 citations across journals including Cell, Science Advances, J Exp Clin Cancer Res, J Neuroinflammation, NPJ Breast Cancer, J Med Chem, Biochem Pharmacol, Anal Chim Acta, Neoplasia, J Cell Physiol, Life Sci Alliance, and eLife. Research fields span virology (SARS-CoV-2, KSHV), cancer (prostate, breast, pancreatic, bladder), neuroinflammation, RNA biology (circRNA, m6A), and protein-protein interactions.
| Species | Application | Title |
|---|---|---|
Cell SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9 | ||
J Exp Clin Cancer Res Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanism. | ||
Sci Adv Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor. | ||
Sci Adv Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor. | ||
J Neuroinflammation Circular RNA METTL9 contributes to neuroinflammation following traumatic brain injury by complexing with astrocytic SND1 | ||
NPJ Breast Cancer PARG inhibition suppresses breast cancer progression and potentiates immunoresponse through MTDH degradation mediated by E3 ligase ITCH-dependent ubiquitination. |
