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SND1 Monoclonal antibody

SND1 Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A6A4

Cat no : 60265-1-Ig

Synonyms

100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11



Tested Applications

Positive WB detected inHepG2 cells, U2OS cells, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas tissue, human breast hyperplasia tissue, human breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60265-1-Ig is SND1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SND1 fusion protein Ag1200
Full Name staphylococcal nuclease and tudor domain containing 1
Calculated molecular weight 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC017180
Gene symbol SND1
Gene ID (NCBI) 27044
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors.

Protocols

Product Specific Protocols
WB protocol for SND1 antibody 60265-1-IgDownload protocol
IHC protocol for SND1 antibody 60265-1-IgDownload protocol
IF protocol for SND1 antibody 60265-1-IgDownload protocol
IP protocol for SND1 antibody 60265-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

J Cell Physiol

The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs.

Authors - Sen Zhang
humanWB

Elife

The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.

Authors - Belinda Baquero-Perez
  • KD Validated
humanIF

Sci Adv

Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.

Authors - Yuan Wang
humanIF, ELISA

Sci Adv

Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.

Authors - Yuan Wang