• Featured Product
  • KD/KO Validated

SNRPA Polyclonal antibody

SNRPA Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10212-1-AP

Synonyms

SNRPA, U1 A, U1 snRNP A, U1A



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, MCF-7 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10212-1-AP targets SNRPA in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity canine, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNRPA fusion protein Ag0283
Full Name small nuclear ribonucleoprotein polypeptide A
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC000405
Gene symbol SNRPA
Gene ID (NCBI) 6626
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP A1 functions in the packaging of nascent RNA polymerase II transcripts and participates in a variety of nuclear and cytoplasmic processes that modulate gene expression. The RNA binding characteristics of hnRNP A1 suggest that it can modulate the expression of specific genes.

Protocols

Product Specific Protocols
WB protocol for SNRPA antibody 10212-1-APDownload protocol
IHC protocol for SNRPA antibody 10212-1-APDownload protocol
IF protocol for SNRPA antibody 10212-1-APDownload protocol
IP protocol for SNRPA antibody 10212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

RNA Biol

Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.

Authors - Sikandar Azam
canineWB

Biochem Biophys Res Commun

Basolateral sorting of the Mg²⁺ transporter CNNM4 requires interaction with AP-1A and AP-1B.

Authors - Yusuke Hirata
  • KD Validated
humanWB,IHC,IF

Cell Prolif

SNRPA enhances tumour cell growth in gastric cancer through modulating NGF expression.

Authors - Ning Dou
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

changwei (Verified Customer) (02-18-2020)

It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa S3
FH

Yaroslav (Verified Customer) (01-06-2020)

Specific signal with modest sensitivity at current dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse ES cells
SNRPA Antibody Western Blot, validation (1:1000 dilution) in mouse ES cells (Cat no:10212-1-AP)