|Positive WB detected in||HEK-293 cells, A431 cells, HeLa cells, MCF-7 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10212-1-AP targets SNRPA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||canine, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SNRPA fusion protein Ag0283|
|Full Name||small nuclear ribonucleoprotein polypeptide A|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC000405|
|Gene ID (NCBI)||6626|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP A1 functions in the packaging of nascent RNA polymerase II transcripts and participates in a variety of nuclear and cytoplasmic processes that modulate gene expression. The RNA binding characteristics of hnRNP A1 suggest that it can modulate the expression of specific genes.
|Product Specific Protocols|
|WB protocol for SNRPA antibody 10212-1-AP||Download protocol|
|IHC protocol for SNRPA antibody 10212-1-AP||Download protocol|
|IF protocol for SNRPA antibody 10212-1-AP||Download protocol|
|IP protocol for SNRPA antibody 10212-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.
Biochem Biophys Res Commun
Basolateral sorting of the Mg²⁺ transporter CNNM4 requires interaction with AP-1A and AP-1B.
SNRPA enhances tumour cell growth in gastric cancer through modulating NGF expression.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
changwei (Verified Customer) (02-18-2020)
It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.
Yaroslav (Verified Customer) (01-06-2020)
Specific signal with modest sensitivity at current dilution