SOD1 Polyclonal antibody

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  WB analysis using 10269-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10269-1-AP

  HeLa (WT and SOD1 KO) lysates prepared with RIPA buffer, 20 μg protein loaded. 10269-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HEK-293 using 10269-1-AP

  WB result of SOD1 antibody (10269-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD1 transfected HEK-293 cells.

• 

  WB analysis using 10269-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10269-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10269-1-AP

  HeLa lysates prepared and IP of SOD1 performed using 2.0 μg of 10269-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of HEK-293 using 10269-1-AP

  IP result of anti-SOD1 (IP:10269-1-AP, 4ug; Detection:10269-1-AP 1:6000) with HEK-293 cells lysate 1470 ug.

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  IHC staining of human liver cancer using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse brain using 10269-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOD1 antibody (10269-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10269-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SOD1 antibody (10269-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 10269-1-AP

  HeLa WT cells (yellow outline) and SOD1 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 10269-1-AP. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• Featured Product
• KD/KO Validated

SOD1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 10269-1-AP

Publications(314)

Reviews (6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Synonyms

ALS1, EC:1.15.1.1, homodimer, hSod1, IPOA

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Tested Applications

• Positive WB detected in: HEK-293 cells, HEK-293T cells, HeLa cells, SH-SY5Y cells, Neuro-2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, Jurkat cells, human placenta tissue
• Positive IP detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human colon tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 281 publications below
• IHC: See 48 publications below
• IF: See 18 publications below
• IP: See 3 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

10269-1-AP targets SOD1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, chicken, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOD1 fusion protein Ag0335
• Full Name: superoxide dismutase 1, soluble
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC001034
• Gene Symbol: SOD1
• Gene ID (NCBI): 6647
• RRID: AB_2193750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00441
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).

Publications

Species	Application	Title
human	IF	Cell Stem Cell; Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons; Authors - Zaida Álvarez
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter
mouse	WB	Bioact Mater; Dynamic degradation patterns of porous polycaprolactone/β-tricalcium phosphate composites orchestrate macrophage responses and immunoregulatory bone regeneration; Authors - Hao Wu
rat	WB	Bioact Mater; A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant; Authors - Yulu Yang
mouse	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	Nat Commun; Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.; Authors - Yun Liao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Scott (Verified Customer) (10-22-2024)

20µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size. Precision plus protein standard ladder #1610373. First 5 lanes are Transgenic SOD1G93A mice, last four lanes are non-transgenic mice.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: SOD1G93A Mouse Brain Lysate

SOD1 Antibody Western Blot validation (1:5000 dilution) in SOD1G93A Mouse Brain Lysate (Cat no:10269-1-AP)

FH

Tianyi (Verified Customer) (08-17-2023)

Paraffin-embedded tissues (4% PFA, 24 h) incubated with SOD1 (1:200) and secondary antibody Alexa Fluor donkey anti-rabbit 594 IgG (H+L).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Ovarian tissue

SOD1 Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:10269-1-AP)

FH

Xingyuan (Verified Customer) (07-17-2023)

Quality was decent.

• Applications: Western Blot
• Primary Antibody Dilution: 1:15000
• Cell Tissue Type: 293T

SOD1 Antibody Western Blot validation (1:15000 dilution) in 293T (Cat no:10269-1-AP)

FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Elise (Verified Customer) (06-24-2022)

Tested on Western blot for 20µg of protein from kidney tissue. Antibody diluted at 1 :1000 in TBS-T BSA 5 %

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Kidney tissue

FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by SOD1 Polyclonal antibody at dilution of 1:100 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: NSC-34 cells