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SOD1 Antibody

SOD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10269-1-AP

Synonyms

ALS, ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse liver tissue, Jurkat cells, human placenta tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human lung tissue, human placenta tissue, human testis tissue, human skin tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10269-1-AP targets SOD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity broiler chick, human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOD1 fusion protein Ag0335
Full Name superoxide dismutase 1, soluble
Calculated molecular weight 16 kDa
Observed molecular weight 16-20 kDa
GenBank accession number BC001034
Gene symbol SOD1
Gene ID (NCBI) 6647
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.

Protocols

Product Specific Protocols
WB protocol for SOD1 antibody 10269-1-APDownload protocol
IHC protocol for SOD1 antibody 10269-1-APDownload protocol
IF protocol for SOD1 antibody 10269-1-APDownload protocol
IP protocol for SOD1 antibody 10269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Toxicol In Vitro

Mechanisms of the immune response cause by cationic and anionic surface functionalized cellulose nanocrystals using cell-based assays.

Authors - Hannah W Despres
ratWB

J Physiol

Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.

Authors - C E Aiken
humanWB

Haematologica

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Authors - Nunziata Maio
ratWB,IHC

Am J Transl Res

Protective effects of resveratrol on random-pattern skin flap survival: an experimental study.

Authors - Jinti Lin
ratWB

Environ Toxicol Pharmacol

Hydrogen sulfide ameliorates disorders in the parafacial respiratory group region of neonatal rats caused by prenatal cigarette smoke exposure via an antioxidative effect.

Authors - Xiang Yan
ratWB

Front Physiol

PMID: 30930790

Authors - Dandan Zhou