Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human placenta tissue, mouse brain tissue, Jurkat cells, rat brain tissue, mouse liver tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver cancer tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10269-1-AP targets SOD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, chicken, goat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SOD1 fusion protein Ag0335|
|Full Name||superoxide dismutase 1, soluble|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||16-20 kDa|
|GenBank accession number||BC001034|
|Gene ID (NCBI)||6647|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).
|Product Specific Protocols|
|WB protocol for SOD1 antibody 10269-1-AP||Download protocol|
|IHC protocol for SOD1 antibody 10269-1-AP||Download protocol|
|IF protocol for SOD1 antibody 10269-1-AP||Download protocol|
|IP protocol for SOD1 antibody 10269-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-17-2021)
Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by SOD1 Polyclonal antibody at dilution of 1:100 showed strong labelling.