|Positive WB detected in||HEK-293 cells, human placenta tissue, mouse brain tissue, Jurkat cells, rat brain tissue, mouse liver tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver cancer tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10269-1-AP targets SOD1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, chicken, goat, human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SOD1 fusion protein Ag0335|
|Full Name||superoxide dismutase 1, soluble|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||16-20 kDa|
|GenBank accession number||BC001034|
|Gene ID (NCBI)||6647|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.
|Product Specific Protocols|
|WB protocol for SOD1 antibody 10269-1-AP||Download protocol|
|IHC protocol for SOD1 antibody 10269-1-AP||Download protocol|
|IF protocol for SOD1 antibody 10269-1-AP||Download protocol|
|IP protocol for SOD1 antibody 10269-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-17-2021)
Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by SOD1 Polyclonal antibody at dilution of 1:100 showed strong labelling.