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  • KD/KO Validated

SOD1 Polyclonal antibody

SOD1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, CoIP, ELISA, IF/ICC

Conjugate

Unconjugated

Cat no : 10269-1-AP

Synonyms

ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, human placenta tissue, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10269-1-AP targets SOD1 in WB, IP, IHC, CoIP, ELISA, IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOD1 fusion protein Ag0335
Full Name superoxide dismutase 1, soluble
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC001034
Gene Symbol SOD1
Gene ID (NCBI) 6647
RRIDAB_2193750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).

Protocols

Product Specific Protocols
WB protocol for SOD1 antibody 10269-1-APDownload protocol
IHC protocol for SOD1 antibody 10269-1-APDownload protocol
IF protocol for SOD1 antibody 10269-1-APDownload protocol
IP protocol for SOD1 antibody 10269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons

Authors - Zaida Álvarez
mouseWB

Cell Metab

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

Authors - Calvin S Carter
mouseWB

Bioact Mater

Dynamic degradation patterns of porous polycaprolactone/β-tricalcium phosphate composites orchestrate macrophage responses and immunoregulatory bone regeneration

Authors - Hao Wu
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
humanWB

Nat Commun

Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.

Authors - Yun Liao
pigWB

Microbiome

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.

Authors - Leli Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tianyi (Verified Customer) (08-17-2023)

Paraffin-embedded tissues (4% PFA, 24 h) incubated with SOD1 (1:200) and secondary antibody Alexa Fluor donkey anti-rabbit 594 IgG (H+L).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Ovarian tissue
SOD1 Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:10269-1-AP)
FH

Xingyuan (Verified Customer) (07-17-2023)

Quality was decent.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:15000
  • Cell Tissue Type: 293T
SOD1 Antibody Western Blot validation (1:15000 dilution) in 293T (Cat no:10269-1-AP)
FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Elise (Verified Customer) (06-24-2022)

Tested on Western blot for 20µg of protein from kidney tissue. Antibody diluted at 1 :1000 in TBS-T BSA 5 %

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Kidney tissue
FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by SOD1 Polyclonal antibody at dilution of 1:100 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: NSC-34 cells