SOD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SOD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

194

Reviews (3)

Cat no : 10269-1-AP

Synonyms

ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]

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Validation Data Gallery

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  WB analysis using 10269-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10269-1-AP

  HeLa (WT and SOD1 KO) lysates prepared with RIPA buffer, 20 μg protein loaded. 10269-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 10269-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10269-1-AP

  WB result of SOD1 antibody (10269-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD1 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 10269-1-AP

  IP Result of anti-SOD1 (IP:10269-1-AP, 3ug; Detection:10269-1-AP 1:1500) with HEK-293 cells lysate 1000ug.

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  IP experiment of HeLa using 10269-1-AP

  HeLa lysates prepared and IP of SOD1 performed using 2.0 μg of 10269-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human liver cancer using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human kidney using 10269-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using SOD1 antibody (10269-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10269-1-AP

  HeLa WT cells (yellow outline) and SOD1 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 10269-1-AP. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IF Staining of HEK-293 using 10269-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10269-1-AP (SOD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, HeLa cells, mouse liver tissue, rat liver tissue, Jurkat cells, SH-SY5Y cells, human placenta tissue, mouse brain tissue
• Positive IP detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 176 publications below
• IHC: See 26 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

10269-1-AP targets SOD1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, canine, chicken, goat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOD1 fusion protein Ag0335
• Full Name: superoxide dismutase 1, soluble
• Calculated molecular weight: 16 kDa
• Observed molecular weight: 16-20 kDa
• GenBank accession number: BC001034
• Gene symbol: SOD1
• Gene ID (NCBI): 6647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).

Publications

Species	Application	Title
human	IF	Cell Stem Cell; Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons; Authors - Zaida Álvarez
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter
human	WB	Nat Commun; Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.; Authors - Yun Liao
pig	WB	Microbiome; Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.; Authors - Leli Wang
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang
human	WB	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Elise (Verified Customer) (06-24-2022)

Tested on Western blot for 20µg of protein from kidney tissue. Antibody diluted at 1 :1000 in TBS-T BSA 5 %

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Kidney tissue

FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by SOD1 Polyclonal antibody at dilution of 1:100 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: NSC-34 cells

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