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SOD1 Antibody 52 Publications

Rabbit Polyclonal| Catalog number: 10269-1-AP

Featured Product KD/KO validated

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Con: 65 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

SOD1 Antibody WB HEK-293 cells 10269-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10269-1-AP(SOD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

SOD1 Antibody WB HEK-293 cells 10269-1-AP

WB result of SOD1 antibody (10269-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD1 transfected HEK-293 cells

SOD1 Antibody IP HEK-293 cells 10269-1-AP

IP Result of anti-SOD1 (IP:10269-1-AP, 3ug; Detection:10269-1-AP 1:1500) with HEK-293 cells lysate 1000ug.

SOD1 Antibody IHC human liver cancer tissue 10269-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP( SOD1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

SOD1 Antibody IHC human liver cancer tissue 10269-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP( SOD1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

SOD1 Antibody IF HEK-293 cells 10269-1-AP

Immunofluorescent analysis of HEK-293 cells using 10269-1-AP(SOD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, broiler chick
Positive WB detected in:
HEK-293 cells, HeLa cells, mouse liver tissue, human placenta tissue, mouse brain tissue, Jurkat cells, rat brain tissue
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human liver cancer tissue, human lung tissue, human placenta tissue, human testis tissue, human skin tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HEK-293 cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SOD1 fusion protein Ag0335
Full name:
superoxide dismutase 1, soluble
Calculated molecular weight:
16 kDa
Observed molecular weight:
16-18 kDa
GenBank accession number:
BC001034
Gene ID (NCBI):
6647
Gene symbol
SOD1
Synonyms
ALS, ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]

Background

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.


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