SOD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SOD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

155

Reviews (1)

Cat no : 10269-1-AP

Synonyms

ALS, ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]

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Validation Data Gallery

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  WB analysis using 10269-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10269-1-AP

  WB result of SOD1 antibody (10269-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10269-1-AP

  WB result of SOD1 antibody (10269-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD1 transfected HEK-293 cells.

• 

  WB analysis of Jurkat using 10269-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10269-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10269-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10269-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10269-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10269-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10269-1-AP

  IP Result of anti-SOD1 (IP:10269-1-AP, 3ug; Detection:10269-1-AP 1:1500) with HEK-293 cells lysate 1000ug.

• 

  IHC staining of human liver cancer using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 10269-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HEK-293 using 10269-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10269-1-AP (SOD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human placenta tissue, mouse brain tissue, Jurkat cells, rat brain tissue, mouse liver tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 142 publications below
• IHC: See 21 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

10269-1-AP targets SOD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, chicken, goat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOD1 fusion protein Ag0335
• Full Name: superoxide dismutase 1, soluble
• Calculated molecular weight: 16 kDa
• Observed molecular weight: 16-20 kDa
• GenBank accession number: BC001034
• Gene symbol: SOD1
• Gene ID (NCBI): 6647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).

Publications

Species	Application	Title
mouse	WB	Toxicol In Vitro; Mechanisms of the immune response cause by cationic and anionic surface functionalized cellulose nanocrystals using cell-based assays.; Authors - Hannah W Despres
rat	WB	J Physiol; Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.; Authors - C E Aiken
human	WB	Haematologica; Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.; Authors - Nunziata Maio
rat	WB,IHC	Am J Transl Res; Protective effects of resveratrol on random-pattern skin flap survival: an experimental study.; Authors - Jinti Lin
rat	WB	Environ Toxicol Pharmacol; Hydrogen sulfide ameliorates disorders in the parafacial respiratory group region of neonatal rats caused by prenatal cigarette smoke exposure via an antioxidative effect.; Authors - Xiang Yan
rat	WB	Front Physiol; PMID: 30930790; Authors - Dandan Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by SOD1 Polyclonal antibody at dilution of 1:100 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: NSC-34 cells

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