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- KD/KO Validated
SOD1 Polyclonal antibody
SOD1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
121
Cat no : 10269-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human placenta tissue, mouse brain tissue, Jurkat cells, rat brain tissue, mouse liver tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 109 publications below |
| IHC | See 16 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
Product Information
10269-1-AP targets SOD1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | bovine, chicken, goat, human, mouse, pig, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SOD1 fusion protein Ag0335 |
| Full Name | superoxide dismutase 1, soluble |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 16-20 kDa |
| GenBank accession number | BC001034 |
| Gene symbol | SOD1 |
| Gene ID (NCBI) | 6647 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IHC protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IF protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IP protocol for SOD1 antibody 10269-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Toxicol In Vitro Mechanisms of the immune response cause by cationic and anionic surface functionalized cellulose nanocrystals using cell-based assays. | ||
J Physiol Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats. | ||
Haematologica Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. | ||
Am J Transl Res Protective effects of resveratrol on random-pattern skin flap survival: an experimental study. | ||
Environ Toxicol Pharmacol Hydrogen sulfide ameliorates disorders in the parafacial respiratory group region of neonatal rats caused by prenatal cigarette smoke exposure via an antioxidative effect. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by SOD1 Polyclonal antibody at dilution of 1:100 showed strong labelling.
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