SPARC Monoclonal antibody

SPARC Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human, rat and More (1)







Cat no : 66426-1-Ig


Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC

Tested Applications

Positive WB detected inA375 cells, human testis tissue, NCCIT cell
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

66426-1-Ig targets SPARC in WB, IF, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SPARC fusion protein Ag7390
Full Name secreted protein, acidic, cysteine-rich (osteonectin)
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 38 kDa, 52 kDa
GenBank Accession NumberBC004974
Gene Symbol SPARC
Gene ID (NCBI) 6678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).


Product Specific Protocols
WB protocol for SPARC antibody 66426-1-IgDownload protocol
IHC protocol for SPARC antibody 66426-1-IgDownload protocol
IF protocol for SPARC antibody 66426-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Cancer

Tissue-resident CXCR4+ macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression

Authors - Zhenyu Liao

Br J Cancer

Inactivation of 3-hydroxybutyrate dehydrogenase type 2 promotes proliferation and metastasis of nasopharyngeal carcinoma by iron retention.

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Front Endocrinol (Lausanne)

Secreted Protein Acidic and Rich in Cysteine Mediates the Development and Progression of Diabetic Retinopathy.

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