SPHK1 Polyclonal antibody

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  WB analysis using 10670-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10670-1-AP (SPHK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 10670-1-AP

  IP result of anti-SPHK1 (IP:10670-1-AP, 4ug; Detection:10670-1-AP 1:300) with L02 cells lysate 2560 ug.

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  IHC staining of human breast cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10670-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of L02 using 10670-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using SPHK1 antibody (10670-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

SPHK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10670-1-AP

Publications(55)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

SPHK, Sphingosine kinase 1, SK1, EC:2.7.1.91, EC:2.3.1.-

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, MCF-7 cells，MDA-MB-231 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 48 publications below
• IHC: See 12 publications below
• IF: See 6 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

10670-1-AP targets SPHK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPHK1 fusion protein Ag1051
• Full Name: sphingosine kinase 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC008040
• Gene Symbol: SPHK1
• Gene ID (NCBI): 8877
• RRID: AB_2195809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYA1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).

Publications

Species	Application	Title
human	WB	Cell Death Discov; SPHK1 enhances olaparib resistance in ovarian cancer through the NFκB/NRF2/ferroptosis pathway; Authors - Kai Teng
mouse	WB	Food Funct; The preventive effect of mussel oil on gestational diabetes mellitus in pregnant mice fed by a high-fat and high-sucrose diet; Authors - Kelei Li
human,mouse	WB,IP	Cells; UCHL1 Regulates Radiation Lung Injury via Sphingosine Kinase-1; Authors - Yulia Epshtein
human	WB	Pharmacol Res; Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.; Authors - Jiale Zhu
human	IHC	Cancer Cell Int; Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer; Authors - Enkui Zhang
human,mouse	WB	Biochem Pharmacol; An inducible sphingosine kinase 1 in hepatic stellate cells potentiates liver fibrosis; Authors - Jin Sol Baek ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (03-12-2020)

Strong and specific band at correct size for western blot.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart