|Positive WB detected in||L02 cells, mouse heart tissue, mouse liver tissue, human kidney tissue, mouse brain tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human kidney tissue, human liver tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17096-1-AP targets SPHK2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SPHK2 fusion protein Ag10569|
|Full Name||sphingosine kinase 2|
|Calculated molecular weight||618 aa, 65 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC010671|
|Gene ID (NCBI)||56848|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SPHK2(Sphingosine kinase 2) is also named as SK2,SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SPHK2 has some isoforms with molecular mass of 65-70 kDa.
|Product Specific Protocols|
|WB protocol for SPHK2 antibody 17096-1-AP||Download protocol|
|IHC protocol for SPHK2 antibody 17096-1-AP||Download protocol|
|IF protocol for SPHK2 antibody 17096-1-AP||Download protocol|
|IP protocol for SPHK2 antibody 17096-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Decreased S1P and SPHK2 are involved in pancreatic acinar cell injury.
An oncogenic role for sphingosine kinase 2.
Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence.
J Biol Chem
Balance between senescence and apoptosis is regulated by telomere damage-induced association between p16 and caspase-3.
Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor T cell response.
Generation of mice with hepatocyte-specific conditional deletion of sphingosine kinase 1.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jason (Verified Customer) (06-09-2021)
Clear staining in mouse liver paraffin section.
David (Verified Customer) (05-04-2021)
It works for cell IF in vitro.
Chen (Verified Customer) (03-13-2021)
Works good for cell immunofluorescence assay in vitro.
Wei (Verified Customer) (03-12-2020)
Sharp and clear band from mouse heart lysates