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SPHK2 Polyclonal antibody
SPHK2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17096-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, mouse brain tissue, human kidney tissue, A549 cells, mouse heart tissue, rat brain tissue, Raji cells, HCT 116 cells, mouse kidney tissue, mouse liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human breast cancer tissue, human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
17096-1-AP targets SPHK2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SPHK2 fusion protein Ag10569 |
Full Name | sphingosine kinase 2 |
Calculated molecular weight | 618 aa, 65 kDa |
Observed molecular weight | 65-70 kDa |
GenBank accession number | BC010671 |
Gene symbol | SPHK2 |
Gene ID (NCBI) | 56848 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SPHK2 antibody 17096-1-AP | Download protocol |
IHC protocol for SPHK2 antibody 17096-1-AP | Download protocol |
IF protocol for SPHK2 antibody 17096-1-AP | Download protocol |
IP protocol for SPHK2 antibody 17096-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Blood Targeting sphingosine kinase 1 induces MCL1 dependent cell death in acute myeloid leukemia. | ||
Bioact Mater Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration. | ||
Oncogene Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma. | ||
PLoS Pathog Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection | ||
Am J Respir Cell Mol Biol Metabolic Adaptation of Airway Smooth Muscle Cells to an SPHK2 Substrate Precedes Cytostasis.
| ||
Oncol Res The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Yasuyo (Verified Customer) (02-23-2023) | Clear staining in mouse kidney paraffin section
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FH Hua (Verified Customer) (01-23-2023) | Good antibody. Works well on WB.
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FH Jason (Verified Customer) (06-09-2021) | Clear staining in mouse liver paraffin section.
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FH David (Verified Customer) (05-04-2021) | It works for cell IF in vitro.
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FH Chen (Verified Customer) (03-13-2021) | Works good for cell immunofluorescence assay in vitro.
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FH Wei (Verified Customer) (03-12-2020) | Sharp and clear band from mouse heart lysates
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