SPHK2 Polyclonal antibody

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  WB analysis using 17096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 17096-1-AP

  WB result of SPHK2 antibody (17096-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPHK2 transfected A549 cells.

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  WB analysis using 17096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 17096-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17096-1-AP

  IP result of anti-SPHK2 (IP:17096-1-AP, 4ug; Detection:17096-1-AP 1:800) with mouse liver tissue lysate 4000ug.

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  IHC staining of human breast cancer using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 17096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 17096-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPHK2 antibody (17096-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 17096-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SPHK2 (17096-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

SPHK2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 17096-1-AP

Publications(48)

Reviews (6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

SPK-2, SPK 2, Sphingosine kinase 2, SK-2, SK 2

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Tested Applications

• Positive WB detected in: L02 cells, mouse brain tissue, human kidney tissue, A549 cells, mouse heart tissue, rat brain tissue, Raji cells, HCT 116 cells, mouse kidney tissue, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human breast cancer tissue, human kidney tissue, human liver tissue, mouse kidney tissue, rat kidney tissue, rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 13 publications below
• WB: See 43 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

17096-1-AP targets SPHK2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPHK2 fusion protein Ag10569
• Full Name: sphingosine kinase 2
• Calculated Molecular Weight: 618 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC010671
• Gene Symbol: SPHK2
• Gene ID (NCBI): 56848
• RRID: AB_10598479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRA0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).

Publications

Species	Application	Title
mouse,rat	WB,IF	Cell Metab; Sphingolipid metabolism controls mammalian heart regeneration; Authors - Xiaoqian Ji ; KD Validated
human	WB	Circ Res; Altered Smooth Muscle Cell Histone Acetylome by the SPHK2/S1P Axis Promotes Pulmonary Hypertension; Authors - A Dushani C U Ranasinghe
human	WB	Blood; Targeting sphingosine kinase 1 induces MCL1 dependent cell death in acute myeloid leukemia.; Authors - Jason A Powell
human,rat	WB,IHC,IF	Bioact Mater; Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.; Authors - Liwen Luo
human	WB,IF,IP	Oncogene; Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.; Authors - Heidi A Neubauer
human	WB	PLoS Pathog; Sphingosine kinase 1/S1P receptor signaling axis is essential for cellular uptake of Neisseria meningitidis in brain endothelial cells; Authors - Ingo Fohmann

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yasuyo (Verified Customer) (02-23-2023)

Clear staining in mouse kidney paraffin section

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: kidney

SPHK2 Antibody Immunohistochemistry validation (1 to 200 dilution) in kidney (Cat no:17096-1-AP)

FH

Hua (Verified Customer) (01-23-2023)

Good antibody. Works well on WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: mouse liver and mouse brain

SPHK2 Antibody Western Blot validation (1:4000 dilution) in mouse liver and mouse brain (Cat no:17096-1-AP)

FH

Jason (Verified Customer) (06-09-2021)

Clear staining in mouse liver paraffin section.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: liver

SPHK2 Antibody Immunohistochemistry validation (1:100 dilution) in liver (Cat no:17096-1-AP)

FH

David (Verified Customer) (05-04-2021)

It works for cell IF in vitro.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: A549 cell line

SPHK2 Antibody Immunofluorescence validation (1:100 dilution) in A549 cell line (Cat no:17096-1-AP)

FH

Chen (Verified Customer) (03-13-2021)

Works good for cell immunofluorescence assay in vitro.

• Applications: Immunofluorescence
• Cell Tissue Type: Heart cells

SPHK2 Antibody Immunofluorescence validation ( dilution) in Heart cells (Cat no:17096-1-AP)

FH

Wei (Verified Customer) (03-12-2020)

Sharp and clear band from mouse heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart