• Featured Product
  • KD/KO Validated

SPHK2 Polyclonal antibody

SPHK2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17096-1-AP

Synonyms

SK 2, sphingosine kinase 2, SPHK2, SPK 2



Tested Applications

Positive WB detected inL02 cells, mouse heart tissue, mouse liver tissue, human kidney tissue, mouse brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, human liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17096-1-AP targets SPHK2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPHK2 fusion protein Ag10569
Full Name sphingosine kinase 2
Calculated molecular weight 618 aa, 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC010671
Gene symbol SPHK2
Gene ID (NCBI) 56848
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPHK2(Sphingosine kinase 2) is also named as SK2,SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SPHK2 has some isoforms with molecular mass of 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for SPHK2 antibody 17096-1-APDownload protocol
IHC protocol for SPHK2 antibody 17096-1-APDownload protocol
IF protocol for SPHK2 antibody 17096-1-APDownload protocol
IP protocol for SPHK2 antibody 17096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biomark Med

Decreased S1P and SPHK2 are involved in pancreatic acinar cell injury.

Authors - Juan Xiao
human

Oncotarget

An oncogenic role for sphingosine kinase 2.

Authors - Heidi A Neubauer
mouseWB

F1000Res

Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence.

Authors - Heidi A Neubauer
  • KO Validated
humanWB,IF,IHC

J Biol Chem

Balance between senescence and apoptosis is regulated by telomere damage-induced association between p16 and caspase-3.

Authors - Shanmugam Panneer Selvam
  • KD Validated
mouseWB

Cell Rep

Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor T cell response.

Authors - Silvia Vaena
  • KO Validated
mouseWB

Transgenic Res

Generation of mice with hepatocyte-specific conditional deletion of sphingosine kinase 1.

Authors - Jinfeng Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jason (Verified Customer) (06-09-2021)

Clear staining in mouse liver paraffin section.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: liver
SPHK2 Antibody Immunohistochemistry validation (1:100 dilution) in liver (Cat no:17096-1-AP)
FH

David (Verified Customer) (05-04-2021)

It works for cell IF in vitro.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: A549 cell line
SPHK2 Antibody Immunofluorescence validation (1:100 dilution) in A549 cell line (Cat no:17096-1-AP)
FH

Chen (Verified Customer) (03-13-2021)

Works good for cell immunofluorescence assay in vitro.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Heart cells
SPHK2 Antibody Immunofluorescence validation ( dilution) in Heart cells (Cat no:17096-1-AP)
FH

Wei (Verified Customer) (03-12-2020)

Sharp and clear band from mouse heart lysates

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart