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  • KD/KO Validated

SPHK2 Polyclonal antibody

SPHK2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 17096-1-AP


SK 2, sphingosine kinase 2, SPHK2, SPK 2

Tested Applications

Positive WB detected inL02 cells, mouse brain tissue, human kidney tissue, A549 cells, mouse heart tissue, rat brain tissue, Raji cells, HCT 116 cells, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

17096-1-AP targets SPHK2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPHK2 fusion protein Ag10569
Full Name sphingosine kinase 2
Calculated molecular weight 618 aa, 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC010671
Gene symbol SPHK2
Gene ID (NCBI) 56848
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).


Product Specific Protocols
WB protocol for SPHK2 antibody 17096-1-APDownload protocol
IHC protocol for SPHK2 antibody 17096-1-APDownload protocol
IF protocol for SPHK2 antibody 17096-1-APDownload protocol
IP protocol for SPHK2 antibody 17096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Targeting sphingosine kinase 1 induces MCL1 dependent cell death in acute myeloid leukemia.

Authors - Jason A Powell

Bioact Mater

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Authors - Liwen Luo


Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.

Authors - Heidi A Neubauer

PLoS Pathog

Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection

Authors - Mengqiong Xu

Am J Respir Cell Mol Biol

Metabolic Adaptation of Airway Smooth Muscle Cells to an SPHK2 Substrate Precedes Cytostasis.

Authors - Pascale Blais-Lecours
  • KD Validated

Oncol Res

The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p.

Authors - Jing Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Yasuyo (Verified Customer) (02-23-2023)

Clear staining in mouse kidney paraffin section

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1 to 200
  • Cell Tissue Type: kidney
SPHK2 Antibody Immunohistochemistry validation (1 to 200 dilution) in kidney (Cat no:17096-1-AP)

Hua (Verified Customer) (01-23-2023)

Good antibody. Works well on WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: mouse liver and mouse brain
SPHK2 Antibody Western Blot validation (1:4000 dilution) in mouse liver and mouse brain (Cat no:17096-1-AP)

Jason (Verified Customer) (06-09-2021)

Clear staining in mouse liver paraffin section.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: liver
SPHK2 Antibody Immunohistochemistry validation (1:100 dilution) in liver (Cat no:17096-1-AP)

David (Verified Customer) (05-04-2021)

It works for cell IF in vitro.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: A549 cell line
SPHK2 Antibody Immunofluorescence validation (1:100 dilution) in A549 cell line (Cat no:17096-1-AP)

Chen (Verified Customer) (03-13-2021)

Works good for cell immunofluorescence assay in vitro.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Heart cells
SPHK2 Antibody Immunofluorescence validation ( dilution) in Heart cells (Cat no:17096-1-AP)

Wei (Verified Customer) (03-12-2020)

Sharp and clear band from mouse heart lysates

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart