SRC Polyclonal antibody

SRC Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Publications

37

Cat no : 11097-1-AP

Synonyms

ASV, c SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

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Validation Data Gallery

• 

  WB analysis of Jurkat using 11097-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 11097-1-AP

  IP Result of anti-SRC (IP:11097-1-AP, 5ug; Detection:11097-1-AP 1:1000) with SH-SY5Y cells lysate 2900ug.

• 

  IHC staining of human breast cancer using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 11097-1-AP

  Immunofluorescent analysis of A431 cells using 11097-1-AP (SRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 11097-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug c-SRC antibody (11097-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: Jurkat cells, A431 cells, CHO cells, COLO 320 cells, mouse brain tissue, SH-SY5Y cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human breast cancer tissue, human brain tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:800
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 28 publications below
• IHC: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11097-1-AP targets SRC in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

• Tested Reactivity: human, mouse, rat, hamster
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRC fusion protein Ag1575
• Full Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Calculated molecular weight: 60 kDa
• Observed molecular weight: 55 kDa, 60 kDa
• GenBank accession number: BC011566
• Gene symbol: SRC
• Gene ID (NCBI): 6714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).

Publications

Species	Application	Title
rat	WB	Aging (Albany NY); DR region specific antibody ameliorated but ouabain worsened renal injury in nephrectomized rats through regulating Na,K-ATPase mediated signaling pathways.; Authors - Juan Wang
human	WB	Front Oncol; SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis.; Authors - Ruoyan Liu
human	WB	Sci Adv; Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.; Authors - Patrick Han
mouse	WB	AMB Express; Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2.; Authors - Xiaomeng Pei
rat	WB	Acta Pharmacol Sin; Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway.; Authors - Cheng-di Liu
human	IHC	Ann Otol Rhinol Laryngol; Hypoxia-Inducible Factor-1α (HIF-1α) Expression on Endothelial Cells in Juvenile Nasopharyngeal Angiofibroma: A Review of 70 cases and Tissue Microarray Analysis.; Authors - Xiaole Song

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