Validation Data Gallery
|Positive WB detected in
|HUVEC cells, mouse brain tissue, COLO 320 cells, Jurkat cells, A431 cells, CHO cells, SH-SY5Y cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human brain tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:3000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:100-1:400
|IF : 1:10-1:100
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11097-1-AP targets c-SRC in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.
|human, mouse, rat, hamster
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|c-SRC fusion protein Ag1575
|v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
|Calculated molecular weight
|Observed molecular weight
|55 kDa, 60 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).
|Product Specific Protocols
|WB protocol for c-SRC antibody 11097-1-AP
|IHC protocol for c-SRC antibody 11097-1-AP
|IF protocol for c-SRC antibody 11097-1-AP
|IP protocol for c-SRC antibody 11097-1-AP
|Click here to view our Standard Protocols
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