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SRC Antibody 24 Publications

Rabbit Polyclonal| Catalog number: 11097-1-AP

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

SRC Antibody WB Jurkat cells 11097-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 11097-1-AP(SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

SRC Antibody IP SH-SY5Y cells 11097-1-AP

IP Result of anti-SRC (IP:11097-1-AP, 5ug; Detection:11097-1-AP 1:1000) with SH-SY5Y cells lysate 2900ug.

SRC Antibody IHC human breast cancer tissue 11097-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP( c-SRC Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

SRC Antibody IHC human breast cancer tissue 11097-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP( c-SRC Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

SRC Antibody IF A431 cells 11097-1-AP

Immunofluorescent analysis of A431 cells using 11097-1-AP(SRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

SRC Antibody FC SH-SY5Y cells 11097-1-AP

1X10^6 SH-SY5Y cells were stained with 0.2ug c-SRC antibody (11097-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, hamster
Positive WB detected in:
Jurkat cells, A431 cells, CHO cells, COLO 320 cells, mouse brain tissue, SH-SY5Y cells
Positive IP detected in:
SH-SY5Y cells
Positive IHC detected in:
human breast cancer tissue, human brain tissue, human spleen tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A431 cells
Positive FC detected in:
SH-SY5Y cells
Recommended dilution:
WB : 1:300-1:800
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:100-1:400
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SRC fusion protein Ag1575
Full name:
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated molecular weight:
60 kDa
Observed molecular weight:
55 kDa, 60 kDa
GenBank accession number:
BC011566
Gene ID (NCBI):
6714
Gene symbol
SRC
Synonyms
ASV, c SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

Background

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).


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