SRC Polyclonal antibody
SRC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, hamster
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 11097-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, A431 cells, CHO cells, COLO 320 cells, mouse brain tissue, SH-SY5Y cells |
Positive IP detected in | SH-SY5Y cells |
Positive IHC detected in | human breast cancer tissue, human brain tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells |
Positive FC detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:300-1:800 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 40 publications below |
IHC | See 8 publications below |
IF | See 1 publications below |
IP | See 3 publications below |
Product Information
11097-1-AP targets SRC in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.
Tested Reactivity | human, mouse, rat, hamster |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SRC fusion protein Ag1575 |
Full Name | v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
Calculated molecular weight | 60 kDa |
Observed molecular weight | 55 kDa, 60 kDa |
GenBank accession number | BC011566 |
Gene symbol | SRC |
Gene ID (NCBI) | 6714 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).
Protocols
Product Specific Protocols | |
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WB protocol for SRC antibody 11097-1-AP | Download protocol |
IHC protocol for SRC antibody 11097-1-AP | Download protocol |
IF protocol for SRC antibody 11097-1-AP | Download protocol |
IP protocol for SRC antibody 11097-1-AP | Download protocol |
FC protocol for SRC antibody 11097-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Aging (Albany NY) DR region specific antibody ameliorated but ouabain worsened renal injury in nephrectomized rats through regulating Na,K-ATPase mediated signaling pathways. | ||
Front Oncol SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis. | ||
Sci Adv Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes. | ||
AMB Express Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2. | ||
Acta Pharmacol Sin Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway. | ||
Ann Otol Rhinol Laryngol Hypoxia-Inducible Factor-1α (HIF-1α) Expression on Endothelial Cells in Juvenile Nasopharyngeal Angiofibroma: A Review of 70 cases and Tissue Microarray Analysis. |