c-SRC Polyclonal antibody

c-SRC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11097-1-AP

Synonyms

ASV, c SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1



Tested Applications

Positive WB detected inHUVEC cells, mouse brain tissue, COLO 320 cells, Jurkat cells, A431 cells, CHO cells, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman breast cancer tissue, human brain tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11097-1-AP targets c-SRC in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity human, mouse, rat, hamster
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-SRC fusion protein Ag1575
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated molecular weight 60 kDa
Observed molecular weight 55 kDa, 60 kDa
GenBank accession numberBC011566
Gene symbol SRC
Gene ID (NCBI) 6714
RRIDAB_2286705
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).

Protocols

Product Specific Protocols
WB protocol for c-SRC antibody 11097-1-APDownload protocol
IHC protocol for c-SRC antibody 11097-1-APDownload protocol
IF protocol for c-SRC antibody 11097-1-APDownload protocol
IP protocol for c-SRC antibody 11097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Genet

Pancreatic cancer risk variant in LINC00673 creates a miR-1231 binding site and interferes with PTPN11 degradation.

Authors - Jian Zheng
mouseWB,IP

Cell Stem Cell

Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.

Authors - Changzheng Li
humanWB

Sci Adv

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Authors - Patrick Han

J Cell Biol

Metalloproteinase MT1-MMP islets act as memory devices for podosome reemergence.

Authors - Karim El Azzouzi
human

Cell Death Differ

The E3 ubiquitin ligase TRIM7 suppressed hepatocellular carcinoma progression by directly targeting Src protein.

Authors - Lihui Zhu
mouseWB

Haematologica

NLRP3 regulates platelet integrin αIIbβ3 outside-in signaling, hemostasis and arterial thrombosis.

Authors - Jianlin Qiao