|Positive WB detected in
|HeLa cells, NIH/3T3 cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17976-1-AP targets SRP54 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|SRP54 fusion protein Ag12166
|signal recognition particle 54kDa
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM). This antibody specifically recognizes the 54kd SRP54 protein.
|Product Specific Protocols
|WB protocol for SRP54 antibody 17976-1-AP
|IHC protocol for SRP54 antibody 17976-1-AP
|IF protocol for SRP54 antibody 17976-1-AP
|IP protocol for SRP54 antibody 17976-1-AP
|Click here to view our Standard Protocols