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  • KD/KO Validated

SSBP1 Polyclonal antibody

SSBP1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 12212-1-AP

Synonyms

Mt SSB, MtSSB, PWP1 interacting protein 17, SSBP, SSBP1



Tested Applications

Positive WB detected inA549 cells, rat pancreas tissue, A375 cells, mouse pancreas tissue
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12212-1-AP targets SSBP1 in WB, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSBP1 fusion protein Ag2860
Full Name single-stranded DNA binding protein 1
Calculated molecular weight 148 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC000895
Gene symbol SSBP1
Gene ID (NCBI) 6742
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase

Protocols

Product Specific Protocols
WB protocol for SSBP1 antibody 12212-1-APDownload protocol
IF protocol for SSBP1 antibody 12212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomes

Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.

Authors - Henrick Horita
humanWB, IHC

J Exp Clin Cancer Res

The circular RNA circZFR phosphorylates Rb promoting cervical cancer progression by regulating the SSBP1/CDK2/cyclin E1 complex.

Authors - Mingyi Zhou
humanWB

PLoS One

Mitochondrial single-stranded DNA binding protein novel de novo SSBP1 mutation in a child with single large-scale mtDNA deletion (SLSMD) clinically manifesting as Pearson, Kearns-Sayre, and Leigh syndromes.

Authors - Margaret A Gustafson
humanWB

J Cancer

Downregulation of Mitochondrial Single Stranded DNA Binding Protein (SSBP1) Induces Mitochondrial Dysfunction and Increases the Radiosensitivity in Non-Small Cell Lung Cancer Cells.

Authors - You Wang
  • KD Validated
mouseWB

Sci Rep

PMID: 28397817

Authors - Xinbin Zhao
humanWB

Oncogenesis

LONP1 and ClpP cooperatively regulate mitochondrial proteostasis for cancer cell survival.

Authors - Yu Geon Lee