SSBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SSBP1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 12212-1-AP

Synonyms

Mt SSB, MtSSB, PWP1 interacting protein 17, SSBP, SSBP1

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Validation Data Gallery

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  WB analysis of A549 using 12212-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis using 12212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 12212-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12212-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12212-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 12212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12212-1-AP (SSBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, A375 cells, mouse pancreas tissue, rat pancreas tissue, Caco-2 cells
• Positive IF detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 20 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• ChIP: See 1 publications below

Product Information

12212-1-AP targets SSBP1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SSBP1 fusion protein Ag2860
• Full Name: single-stranded DNA binding protein 1
• Calculated Molecular Weight: 148 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC000895
• Gene Symbol: SSBP1
• Gene ID (NCBI): 6742
• RRID: AB_2195320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase

Publications

Species	Application	Title
human	WB	Cell; MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.; Authors - Aindrila Chatterjee
human	WB	Nat Cell Biol; Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.; Authors - Timo Rey
human	WB	Mol Cell; Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion.; Authors - Aaron F Phillips
		Sci Adv; The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.; Authors - Min Jiang ; KO Validated
human	IHC	Nucleic Acids Res; Top3α is the replicative topoisomerase in mitochondrial DNA replication.; Authors - Anu Hangas
human	WB	Brain; Heterozygous SSBP1 start loss mutation co-segregates with hearing loss and the m.1555A>G mtDNA variant in a large multigenerational family.; Authors - Peter J Kullar