ST5 Polyclonal antibody

• 

  WB analysis using 30996-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30996-1-AP (ST5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

ST5 Polyclonal Antibody for WB, ELISA

Cat No. 30996-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DENN domain-containing protein 2B, DENND2B, HTS1

---

Tested Applications

• Positive WB detected in: A431 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30996-1-AP targets ST5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ST5 fusion protein Ag34032
• Full Name: suppression of tumorigenicity 5
• Calculated Molecular Weight: 1137 aa, 126 kDa
• Observed Molecular Weight: 120-150 kDa
• GenBank Accession Number: BC036655
• Gene Symbol: ST5
• Gene ID (NCBI): 6764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78524
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST5, also known as DEN2B (DENN domain-containing protein 2B), has three isoforms with molecular weights of 126 kDa, 82 kDa, and 70 kDa, respectively. The mainstream molecular weight is 126kd. ST5 is widely expressed except in peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial cells and fibroblasts (including tumorigenic cells), but not in lymphoid cell lines (at the protein level).