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  • KD/KO Validated

STAM Polyclonal antibody

STAM Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12434-1-AP

Synonyms

STAM, STAM 1, STAM1



Tested Applications

Positive WB detected inJurkat cells, Multi-cells , Y79 cells, mouse brain tissue
Positive IP detected inPC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12434-1-AP targets STAM in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAM fusion protein Ag3112
Full Name signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
Calculated molecular weight 59 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC030586
Gene symbol STAM
Gene ID (NCBI) 8027
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)

Protocols

Product Specific Protocols
WB protocol for STAM antibody 12434-1-APDownload protocol
IHC protocol for STAM antibody 12434-1-APDownload protocol
IF protocol for STAM antibody 12434-1-APDownload protocol
IP protocol for STAM antibody 12434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Biol Chem

The α-Arrestin ARRDC3 Regulates the Endosomal Residence Time and Intracellular Signaling of the β2-Adrenergic Receptor.

Authors - Xufan Tian
HUMANWB

J Biol Chem

β-arrestin1 and signal-transducing adaptor molecule 1 (STAM1) cooperate to promote focal adhesion kinase autophosphorylation and chemotaxis via the chemokine receptor CXCR4.

Authors - Olga Alekhina
  • KD Validated
humanWB,IF

Mol Biol Cell

Arrestin-2 interacts with the endosomal sorting complex required for transport machinery to modulate endosomal sorting of CXCR4.

Authors - Malik Rohit R
  • KD Validated
humanWB,IF

Mol Biol Cell

The ubiquitin ligase Deltex-3L regulates endosomal sorting of the G protein-coupled receptor CXCR4.

Authors - Justine Holleman
humanWB

J Biol Chem

The Endosomal Sorting Complex Required for Transport Pathway Mediates Chemokine Receptor CXCR4 Promoted Lysosomal Degradation of the mTOR Antagonist DEPTOR.

Authors - Rita Verma
  • KD Validated
humanWB,IF

J Biol Chem

Novel roles for the E3 ubiquitin ligase atrophin-interacting protein 4 and signal transduction adaptor molecule 1 in G protein-coupled receptor signaling.

Authors - Malik Rohit R