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STAT3 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Activ Motif logo

Cat No : 61645,61646 61645

Synonyms

STAT3, Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor), APRF, Acute-Phase Response Factor, HIES, DNA-Binding Protein APRF, Signal Transducer And Activator Of Transcription 3, jak-stat, transcription activator, antibody, antibodies, polyclonal, pab, western blotting, wb, sample

Validation Data Gallery

  • STAT3 antibody (pAb) tested by Western blot. Detection of STAT3 by Western blot analysis. Nuclear extract of HepG2 cells (30 ug) probed with STAT3 antibody at a dilution of 1:2,000.

    STAT3 antibody (pAb) tested by Western blot. Detection of STAT3 by Western blot analysis. Nuclear extract of HepG2 cells (30 ug) probed with STAT3 antibody at a dilution of 1:2,000.

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB*: 1:1,000 - 1:2,500 *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human STAT3.
Full Name STAT3 antibody (pAb)
Synonyms STAT3, Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor), APRF, Acute-Phase Response Factor, HIES, DNA-Binding Protein APRF, Signal Transducer And Activator Of Transcription 3, jak-stat, transcription activator, antibody, antibodies, polyclonal, pab, western blotting, wb, sample
Molecular weight 96 kDa
GenBank accession numberNP_644805
RRIDAB_2793718
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

STATs (signal transducers and activators of transcription) are a family of 7 transcription factors that form part of the JAK-STAT signaling cascade. This cascade is the basis of the signal transduction mechanism for many cytokine receptors. Once activated by phosphorylation by JAKs, STATs translocate to the nucleus. Accumulation of STATs in the nucleus is both rapid and tightly controlled. A number of factors regulate the JAK-STAT pathway including STAT dephosphorylation by phosphatases, altered nuclear import-export dynamics of STAT, and STAT gene activation antagonists such as SOCS (suppressors of cytokine signaling) and PIAS (Protein Inhibitors of Activated STATs).