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STAT5A antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

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Cat No : 61133,61134 61133

Synonyms

STAT 5A, STAT5A

Validation Data Gallery

  • STAT5A antibody (pAb) tested by Immunoprecipitation. 10 ug of STAT5A antibody was used to immunoprecipitate STAT5A from 400 ug of K562 cytosolic cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the STAT5A antibody at a dilution of 1 ug/ml.

    STAT5A antibody (pAb) tested by Immunoprecipitation. 10 ug of STAT5A antibody was used to immunoprecipitate STAT5A from 400 ug of K562 cytosolic cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the STAT5A antibody at a dilution of 1 ug/ml.

  • STAT5A antibody (pAb) tested by Western blot. STAT5A antibody detection by Western blot. The analysis was performed using K-562 cytosolic-cell extract (25 ug) and STAT5A antibody (pAb) at a 1 ug/ml dilution.

    STAT5A antibody (pAb) tested by Western blot. STAT5A antibody detection by Western blot. The analysis was performed using K-562 cytosolic-cell extract (25 ug) and STAT5A antibody (pAb) at a 1 ug/ml dilution.

Product Information

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ug per IP WB: 0.5 - 2 ug/ml dilution
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This STAT5A antibody was raised against a peptide in the C-terminal region of human STAT5A.
Full Name STAT5A antibody (pAb)
Synonyms STAT 5A, STAT5A
Molecular weight 92 kDa
GenBank accession numberNP_003143
RRIDAB_2793520
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

STAT5A and STAT5B are encoded by separate genes but are 90% identical. They mediate gene expression in response to IL-2, IL-3, IL-7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. The STAT5A/B signaling pathway is involved in the transition of organ-confined prostate cancer to hormone-refractory disease. This implies that the active JAK2-STAT5A/B signaling pathway potentially provides the means for pharmacological intervention of clinical prostate cancer progression.