STEAP3 Polyclonal antibody

STEAP3 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 17186-1-AP

Synonyms

dudlin 2, Dudulin 2, hpHyde, hTSAP6, Metalloreductase STEAP3, pHyde, STEAP family member 3, STEAP3, STMP3, TSAP6

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Validation Data Gallery

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  WB analysis of HepG2 using 17186-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17186-1-AP (STEAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17186-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17186-1-AP (STEAP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 17186-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 17186-1-AP (STEAP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17186-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17186-1-AP (STEAP3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17186-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17186-1-AP (STEAP3 antibody) at dilution of 1:100 (under 40x lens).

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

17186-1-AP targets STEAP3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STEAP3 fusion protein Ag10970
• Full Name: STEAP family member 3
• Calculated Molecular Weight: 488 aa, 55 kDa
• GenBank Accession Number: BC042150
• Gene Symbol: STEAP3
• Gene ID (NCBI): 55240
• RRID: AB_2197841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.; Authors - Steinberg Florian F
human	WB	Nat Commun; Regulation of the endosomal SNX27-retromer by OTULIN.; Authors - Aurelia Stangl
mouse	WB	J Exp Med; Ferrous iron-activatable drug conjugate achieves potent MAPK blockade in KRAS-driven tumors.; Authors - Honglin Jiang
mouse	WB	Int J Biol Macromol; Exosomes from tannic acid-stimulated macrophages accelerate wound healing through miR-221-3p mediated fibroblasts migration by targeting CDKN1b; Authors - Xu Song
human	WB	J Nucl Med; Ferronostics: Measuring Tumoral Ferrous Iron with PET to Predict Sensitivity to Iron-Targeted Cancer Therapies.; Authors - Ning Zhao
mouse	WB	Blood Adv; Differences in Steap3 expression are a mechanism of genetic variation of RBC storage and oxidative damage in mice.; Authors - Heather L Howie