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  • KD/KO Validated

STMN2 Polyclonal antibody

STMN2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10586-1-AP

Synonyms

Protein SCG10, SCG10, SCGN10, SGC10, Stathmin 2, stathmin like 2, STMN2



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10586-1-AP targets STMN2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STMN2 fusion protein Ag0929
Full Name stathmin-like 2
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC006302
Gene symbol STMN2
Gene ID (NCBI) 11075
RRIDAB_2197283
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

Protocols

Product Specific Protocols
WB protocol for STMN2 antibody 10586-1-APDownload protocol
IHC protocol for STMN2 antibody 10586-1-APDownload protocol
IF protocol for STMN2 antibody 10586-1-APDownload protocol
IP protocol for STMN2 antibody 10586-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuron

Molecular substrates of altered axonal growth and brain connectivity in a mouse model of schizophrenia.

Authors - Jun Mukai
mouseWB

Proc Natl Acad Sci U S A

SCG10 is a JNK target in the axonal degeneration pathway.

Authors - Shin Jung Eun JE
  • KD Validated
mouseWB

J Cell Biol

SARM1 acts downstream of neuroinflammatory and necroptotic signaling to induce axon degeneration.

Authors - Kwang Woo Ko
mouseWB

J Cell Biol

A neuroprotective agent that inactivates prodegenerative TrkA and preserves mitochondria.

Authors - Konstantin Feinberg
zebrafishIHC

Elife

Retrograde Ret signaling controls sensory pioneer axon outgrowth.

Authors - Adam Tuttle
mouseIHC,IF

J Neurosci

CNGA3: a target of spinal nitric oxide/cGMP signaling and modulator of inflammatory pain hypersensitivity.

Authors - Heine Sandra S

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tatyana (Verified Customer) (01-09-2023)

Good signal both in WB and ICC on neuroblastoma cells (neuronal cell line). Was used 1:1000 in both applications. Gave some non-specific bands in WB. ICC - 5% goat serum / PBST WB - 4% milk / TBST

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human / SH-SY5Y cells
STMN2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:10586-1-AP)