|Positive WB detected in||rat brain tissue, mouse cerebellum tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human medulloblastoma tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells, mouse brain tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10586-1-AP targets STMN2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||STMN2 fusion protein Ag0929|
|Full Name||stathmin-like 2|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||18-21 kDa|
|GenBank accession number||BC006302|
|Gene ID (NCBI)||11075|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)
|Product Specific Protocols|
|WB protocol for STMN2 antibody 10586-1-AP||Download protocol|
|IHC protocol for STMN2 antibody 10586-1-AP||Download protocol|
|IF protocol for STMN2 antibody 10586-1-AP||Download protocol|
|IP protocol for STMN2 antibody 10586-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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