Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue, Neuro-2a cells, PC-12 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10586-1-AP targets STMN2 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0929 Product name: Recombinant human STMN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-179 aa of BC006302 Sequence: MAKTAMAYKEKMKELSMLSLICSCFYPEPRNINIYTYDDMEVKQINKRASGQAFELILKPPSPISEAPRTLASPKKKDLSLEEIQKKLEAAEERRKSQEAQVLKQLAEKREHEREVLQKALEENNNFSKMAEEKLILKMEQIKENREANLAAIIERLQEKERHAAEVRRNKELQVELSG Predict reactive species |
| Full Name | stathmin-like 2 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 18-21 kDa |
| GenBank Accession Number | BC006302 |
| Gene Symbol | STMN2 |
| Gene ID (NCBI) | 11075 |
| RRID | AB_2197283 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q93045 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STMN2 antibody 10586-1-AP | Download protocol |
| IHC protocol for STMN2 antibody 10586-1-AP | Download protocol |
| IP protocol for STMN2 antibody 10586-1-AP | Download protocol |
| WB protocol for STMN2 antibody 10586-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-STMN2 Polyclonal Antibody (Cat# 10586-1-AP) has 53 citations published in journals including Nature, Science, Cell, Neuron, Brain, PNAS, J Cell Biol, Cell Reports, Autophagy, Neurobiology of Disease, Pain, and others. Associated research fields encompass neurodegeneration (ALS, FTD, SMA), axon regeneration, Wallerian degeneration, neuropathic pain, peripheral nerve injury and repair, neuroinflammation, mitochondrial dysfunction, and oncology (lung cancer, glioma).
| Species | Application | Title |
|---|---|---|
Cell A glycolytic shunt via the pentose phosphate pathway is a metabolic checkpoint for nervous system sensory homeostasis and axonal regeneration. | ||
Autophagy Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations. | ||
Neuron Molecular substrates of altered axonal growth and brain connectivity in a mouse model of schizophrenia. | ||
Adv Sci (Weinh) Pathophysiology-Directed Engineering of a Combination Nanoanalgesic for Neuropathic Pain |
Reviews
What customers say
All three reviews are positive, with ratings of 4–5 stars. Users consistently applied a 1:1000 dilution across Western Blot and Immunofluorescence applications on liver tissue, human brain cortical tissue, and SH-SY5Y cells. Reviewers reported strong, reliable signals in both WB and ICC. One user noted occasional non-specific bands in WB but still rated the product highly overall. No major issues were raised; the antibody is described as "very promising" and well-suited for neuronal cell studies.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-13-2025) | good for western blot
|
Daipayan (Verified Customer) (10-23-2025) | Very promising product
|
Tatyana (Verified Customer) (01-09-2023) | Good signal both in WB and ICC on neuroblastoma cells (neuronal cell line). Was used 1:1000 in both applications. Gave some non-specific bands in WB. ICC - 5% goat serum / PBST WB - 4% milk / TBST
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