STMN2 Monoclonal antibody

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  WB analysis of PC-12 using 67204-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 67204-1-Ig

  WB result of STMN2 antibody (67204-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STMN2 transfected SH-SY5Y cells.

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  WB analysis of fetal human brain using 67204-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67204-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 67204-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 67204-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67204-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67204-1-Ig (STMN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67204-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67204-1-Ig (STMN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 67204-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody (67204-1-Ig, Clone: 1F6C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

STMN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67204-1-Ig

Clone No.1F6C4

Publications(3)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

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Tested Applications

• Positive WB detected in: PC-12 cells, fetal human brain tissue, mouse brain tissue, pig brain tissue, rat brain tissue, SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

67204-1-Ig targets STMN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STMN2 fusion protein Ag28990
• Full Name: stathmin-like 2
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC006302
• Gene Symbol: STMN2
• Gene ID (NCBI): 11075
• RRID: AB_2882497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93045
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STMN2 gene encodes a SCG10 (superior cervical ganglion-10) protein, a regulator of microtubule stability. When phosphorylated by MAPK8, SCG10 stabilizes microtubules and consequently controls neurite length in cortical neurons. SCG10 was early considered a neuron-specific growth-associated protein in Alzheimer's disease. In the developing brain, SCG10 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.

Publications

Species	Application	Title
human	IF	Nature; A model of human neural networks reveals NPTX2 pathology in ALS and FTLD; Authors - Marian Hruska-Plochan
human	IF	Neuron; TDP-43 seeding induces cytoplasmic aggregation heterogeneity and nuclear loss of function of TDP-43; Authors - Jens Rummens
human	WB	bioRxiv; Tubulin Regulates the Stability and Localization of STMN2 by Binding Preferentially to Its Soluble Form; Authors - Xiang Deng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (06-15-2025)

Incubation ON at 4C, in 5% goat serum/PBST. Gives specific cytoplasmic/Golgi signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y cells/human

STMN2 Antibody Immunofluorescence validation (1:1000 dilution) in SH-SY5Y cells/human (Cat no:67204-1-Ig)