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Syntaxin 12 Polyclonal antibody

Syntaxin 12 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14259-1-AP

Synonyms

STX12, STX13, STX14, syntaxin 12



Tested Applications

Positive WB detected inmouse brain tissue, BxPC-3 cells, human brain tissue, human heart tissue, mouse heart tissue
Positive IHC detected inhuman lung cancer tissue, human brain tissue, human kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14259-1-AP targets Syntaxin 12 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntaxin 12 fusion protein Ag5532
Full Name syntaxin 12
Calculated molecular weight 32 kDa
Observed molecular weight 33 kDa, 39 kDa, 66 kDa
GenBank accession numberBC046999
Gene symbol STX12
Gene ID (NCBI) 23673
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).

Protocols

Product Specific Protocols
WB protocol for Syntaxin 12 antibody 14259-1-APDownload protocol
IHC protocol for Syntaxin 12 antibody 14259-1-APDownload protocol
IF protocol for Syntaxin 12 antibody 14259-1-APDownload protocol
FC protocol for Syntaxin 12 antibody 14259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem J

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.

Authors - Nazma Malik
humanWB,IF

Mol Cell

Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.

Authors - Lu Yubing Y
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