|Positive WB detected in||mouse brain tissue, BxPC-3 cells, human brain tissue, human heart tissue, mouse heart tissue|
|Positive IHC detected in||human lung cancer tissue, human brain tissue, human kidney tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14259-1-AP targets Syntaxin 12 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Syntaxin 12 fusion protein Ag5532|
|Full Name||syntaxin 12|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||33 kDa, 39 kDa, 66 kDa|
|GenBank accession number||BC046999|
|Gene ID (NCBI)||23673|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).
|Product Specific Protocols|
|WB protocol for Syntaxin 12 antibody 14259-1-AP||Download protocol|
|IHC protocol for Syntaxin 12 antibody 14259-1-AP||Download protocol|
|IF protocol for Syntaxin 12 antibody 14259-1-AP||Download protocol|
|FC protocol for Syntaxin 12 antibody 14259-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.
Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.