Syntaxin 12 Polyclonal antibody

• Featured Product
• KD/KO Validated

Syntaxin 12 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14259-1-AP

Synonyms

STX12, STX13, STX14, syntaxin 12

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Validation Data Gallery

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  IF Staining of HepG2 using 14259-1-AP

  Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 14259-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Syntaxin 12 antibody (14259-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

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  WB analysis of mouse brain using 14259-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 14259-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 14259-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, BxPC-3 cells, human brain tissue, human heart tissue, mouse heart tissue
• Positive IHC detected in: human lung cancer tissue, human brain tissue, human kidney tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

14259-1-AP targets Syntaxin 12 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Syntaxin 12 fusion protein Ag5532
• Full Name: syntaxin 12
• Calculated molecular weight: 32 kDa
• Observed molecular weight: 33 kDa, 39 kDa, 66 kDa
• GenBank accession number: BC046999
• Gene symbol: STX12
• Gene ID (NCBI): 23673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).

Publications

Species	Application	Title
human	WB	Biochem J; Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.; Authors - Nazma Malik
human	WB,IF	Mol Cell; Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.; Authors - Lu Yubing Y ; KD Validated