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Syntaxin 12 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 14259-1-AP

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Con: 25 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

Syntaxin 12 Antibody IF HepG2 cells 14259-1-AP

Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Syntaxin 12 Antibody FC HepG2 cells 14259-1-AP

1X10^6 HepG2 cells were stained with 0.2ug Syntaxin 12 antibody (14259-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

Syntaxin 12 Antibody WB mouse brain tissue 14259-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP(Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Syntaxin 12 Antibody IHC human lung cancer tissue 14259-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 14259-1-AP(Syntaxin 12 antibody) at dilution of 1:100 (under 10x lens)

Syntaxin 12 Antibody IHC human lung cancer tissue 14259-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 14259-1-AP(Syntaxin 12 antibody) at dilution of 1:100 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, BxPC-3 cells, human brain tissue, human heart tissue, mouse heart tissue
Positive IHC detected in:
human lung cancer tissue, human brain tissue, human kidney tissue, human pancreas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Syntaxin 12 fusion protein Ag5532
Full name:
syntaxin 12
Calculated molecular weight:
32 kDa
Observed molecular weight:
33 kDa,39 kDa,66 kDa
GenBank accession number:
BC046999
Gene ID (NCBI):
23673
Gene symbol
STX12
Synonyms
STX12, STX13, STX14, syntaxin 12

Background

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).


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