Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, human heart tissue, BxPC-3 cells, mouse heart tissue, mouse brain tissue, mouse kidney tissue |
| Positive IHC detected in | human lung cancer tissue, human kidney tissue, mouse brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 2 publications below |
Product Information
14259-1-AP targets Syntaxin 12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Syntaxin 12 fusion protein Ag5532 Predict reactive species |
| Full Name | syntaxin 12 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 33-39 kDa, 66 kDa |
| GenBank Accession Number | BC046999 |
| Gene Symbol | Syntaxin 12 |
| Gene ID (NCBI) | 23673 |
| RRID | AB_2198222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86Y82 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Syntaxin 12 antibody 14259-1-AP | Download protocol |
| IHC protocol for Syntaxin 12 antibody 14259-1-AP | Download protocol |
| IF protocol for Syntaxin 12 antibody 14259-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.
| ||
PLoS Biol Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. | ||
Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. | ||
J Biol Chem Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome | ||
Mol Biol Cell Rab11 regulates autophagy at dendritic spines in an mTOR- and NMDA-dependent manner | ||
Thorac Cancer SLC34A2 promotes cell proliferation by activating STX17-mediated autophagy in esophageal squamous cell carcinoma |
