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Syntaxin 12 Polyclonal antibody
Syntaxin 12 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 14259-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, BxPC-3 cells, human brain tissue, human heart tissue, mouse heart tissue |
Positive IHC detected in | human lung cancer tissue, human brain tissue, human kidney tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IF | See 1 publications below |
Product Information
14259-1-AP targets Syntaxin 12 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Syntaxin 12 fusion protein Ag5532 |
Full Name | syntaxin 12 |
Calculated molecular weight | 32 kDa |
Observed molecular weight | 33 kDa, 39 kDa, 66 kDa |
GenBank accession number | BC046999 |
Gene symbol | STX12 |
Gene ID (NCBI) | 23673 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).
Protocols
Product Specific Protocols | |
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WB protocol for Syntaxin 12 antibody 14259-1-AP | Download protocol |
IHC protocol for Syntaxin 12 antibody 14259-1-AP | Download protocol |
IF protocol for Syntaxin 12 antibody 14259-1-AP | Download protocol |
FC protocol for Syntaxin 12 antibody 14259-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. | ||
Mol Cell Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.
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