SULT4A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SULT4A1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 12578-1-AP

Synonyms

BR STL 1, BRSTL1, hBR STL, hBR STL 1, NST, ST4A1, Sulfotransferase 4A1, SULT4A1, SULTX3

---

Validation Data Gallery

• 

  WB analysis using 12578-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12578-1-AP (SULT4A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12578-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12578-1-AP (SULT4A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12578-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12578-1-AP (SULT4A1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12578-1-AP

  IP result of anti-SULT4A1 (IP:12578-1-AP, 4ug; Detection:12578-1-AP 1:300) with mouse brain tissue lysate 4000ug.

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

12578-1-AP targets SULT4A1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SULT4A1 fusion protein Ag3271
• Full Name: sulfotransferase family 4A, member 1
• Calculated Molecular Weight: 284 aa, 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC022459
• Gene Symbol: SULT4A1
• Gene ID (NCBI): 25830
• RRID: AB_2197933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULT4A1, also named as SULTX3, NST, ST4A1, hBR-STL and hBR-STL-1, belongs to the sulfotransferase 1 family. It may catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. SULT4A1 displays activity towards L-triiodothyronine, thyroxine, estrone, p-nitrophenol, 2-naphtylamine, and 2-naphtol. It may have a role in the sulfation of drugs and neurotransmitters in the CNS. SULT4A1 is a brain-specific sulfotransferase believed to be involved in the metabolism of neurotransmitters. It is a novel cytosolic sulfotransferase that is primarily expressed in the brain.

Publications

Species	Application	Title
mouse,rat	WB,IF	J Neurosci; SULT4A1 Modulates Synaptic Development and Function by Promoting the Formation of PSD-95/NMDAR Complex.; Authors - Lorenza Culotta ; KD Validated
human,rat	WB,IHC	Biochem Pharmacol; Identification of metabolic pattern and bioactive form of resveratrol in human medulloblastoma cells.; Authors - Shu Xiao-Hong XH
mouse	WB	Mol Pharmacol; Regulation of mouse brain-selective sulfotransferase sult4a1 by cAMP response element-binding protein and activating transcription factor-2.; Authors - Butcher Neville J NJ
human	WB	FEBS J; Distinct sulfonation activities in resveratrol-sensitive and resveratrol-insensitive human glioblastoma cells.; Authors - Sun Zheng Z
	WB	Sci Rep; Sulfotransferase 4A1 activity facilitates sulfate-dependent cellular protection to oxidative stress.; Authors - Evan J Brettrager
human	WB,IHC,IF	PLoS One; Metabolic patterns and biotransformation activities of resveratrol in human glioblastoma cells: relevance with therapeutic efficacies.; Authors - Shu Xiao-Hong XH