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  • KD/KO Validated

SURVIVIN Monoclonal antibody

SURVIVIN Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human and More (3)







Cat no : 66495-1-Ig


API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, BIRC5, EPR 1, IAP4, SURVIVIN

Tested Applications

Positive WB detected inHEK-293 cells, Raji cells, K-562 cells, HeLa cells, A431 cells, U-937 cells, Jurkat cells
Positive FC detected inJurkat cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66495-1-Ig targets SURVIVIN in WB,FC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SURVIVIN fusion protein Ag20958
Full Name baculoviral IAP repeat-containing 5
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC008718
Gene Symbol BIRC5
Gene ID (NCBI) 332
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.


Product Specific Protocols
WB protocol for SURVIVIN antibody 66495-1-IgDownload protocol
FC protocol for SURVIVIN antibody 66495-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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