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SURVIVIN Monoclonal antibody

SURVIVIN Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

3B9H7

Cat no : 66495-1-Ig

Synonyms

API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, BIRC5, EPR 1, IAP4, SURVIVIN



Tested Applications

Positive WB detected inHEK-293 cells, Raji cells, K-562 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

66495-1-Ig targets SURVIVIN in WB, IF, FC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SURVIVIN fusion protein Ag20958
Full Name baculoviral IAP repeat-containing 5
Calculated molecular weight 16 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC008718
Gene symbol BIRC5
Gene ID (NCBI) 332
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

Protocols

Product Specific Protocols
WB protocol for SURVIVIN antibody 66495-1-IgDownload protocol
FC protocol for SURVIVIN antibody 66495-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Acta Biochim Biophys Sin (Shanghai)

Proliferation inhibition and apoptosis promotion by dual silencing of VEGF and Survivin in human osteosarcoma.

Authors - Junquan Gu
  • KD Validated