SUZ12 Polyclonal antibody

SUZ12 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20366-1-AP

Synonyms

ChET 9 protein, CHET9, JJAZ1, Joined to JAZF1 protein, KIAA0160, Polycomb protein SUZ12, SUZ12



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

20366-1-AP targets SUZ12 in WB, IP, IHC,ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name suppressor of zeste 12 homolog (Drosophila)
Calculated molecular weight 83 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberNM_015355
Gene symbol SUZ12
Gene ID (NCBI) 23512
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.

Protocols

Product Specific Protocols
WB protocol for SUZ12 antibody 20366-1-APDownload protocol
IHC protocol for SUZ12 antibody 20366-1-APDownload protocol
IP protocol for SUZ12 antibody 20366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Transl Oncol

TRPS1 Suppresses Breast Cancer Epithelial-mesenchymal Transition Program as a Negative Regulator of SUZ12.

Authors - Jing Hu
humanWB

Acta Pharmacol Sin

AZD9291 inactivates the PRC2 complex to mediate tumor growth inhibition.

Authors - Kai-Li Zhang
humanWB

Sci Rep

Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.

Authors - Masamitsu N Asaka
humanWB

EMBO J

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

Authors - Xu Wang