SUZ12 Polyclonal antibody

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  WB analysis using 20366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20366-1-AP (SUZ12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20366-1-AP

  WB result of SUZ12 antibody (20366-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUZ12 transfected HEK-293 cells.

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  IP experiment of HeLa using 20366-1-AP

  IP result of anti-SUZ12 (IP:20366-1-AP, 4ug; Detection:20366-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IP experiment of HeLa using 20366-1-AP

  IP result of anti-SUZ12 (IP:20366-1-AP, 4ug; Detection:20366-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:400.

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  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:100.

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  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:100.

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  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:200.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:200.

• 

  IHC staining of human breast cancer using 20366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20366-1-AP (SUZ12 Antibody) at dilution of 1:400.

• Featured Product
• KD/KO Validated

SUZ12 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 20366-1-AP

Publications(20)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IP, CoIP, ELISA

Synonyms

ChET 9 protein, CHET9, JJAZ1, Joined to JAZF1 protein, KIAA0160

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

20366-1-AP targets SUZ12 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: suppressor of zeste 12 homolog (Drosophila)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: NM_015355
• Gene Symbol: SUZ12
• Gene ID (NCBI): 23512
• RRID: AB_10694152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15022
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human	WB	Mol Cell; TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.; Authors - Iva A Tchasovnikarova ; KD Validated
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang
human	WB	Eur J Med Chem; Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.; Authors - Cheng Wang
human	WB	Sci Rep; Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.; Authors - Masamitsu N Asaka
human	WB	Am J Cancer Res; N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex.; Authors - Jing Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikhil (Verified Customer) (01-07-2022)

HCT116 whole cell extract, 20micro/lane. Lane 2-4 transient expression of EZH2

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116

SUZ12 Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:20366-1-AP)