Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20366-1-AP targets SUZ12 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | suppressor of zeste 12 homolog (Drosophila) |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 80-90 kDa |
| GenBank Accession Number | NM_015355 |
| Gene Symbol | SUZ12 |
| Gene ID (NCBI) | 23512 |
| RRID | AB_10694152 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15022 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SUZ12 antibody 20366-1-AP | Download protocol |
| IP protocol for SUZ12 antibody 20366-1-AP | Download protocol |
| WB protocol for SUZ12 antibody 20366-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-EZH2 antibody (Cat# 20366-1-AP) has 21 total citations published in journals including Nature Communications, Molecular Cancer, Molecular Cell, EMBO Journal, Advanced Science, Cell Death & Disease, J Exp Clin Cancer Res, Int J Biol Sci, Acta Pharmacol Sin, Eur J Med Chem, Cell Mol Life Sci, Front Pharmacol, Inflammation, mSystems, Transl Oncol, Sci Rep, Cell Signal, Iran J Basic Med Sci, Am J Cancer Res, and Photochem Photobiol. Research fields span epigenetics (PRC2/H3K27me3 regulation), cancer biology (ovarian, colon, breast, bladder, lung, glioblastoma), senescence, inflammation, and infectious disease.
| Species | Application | Title |
|---|---|---|
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Nat Commun Phosphorylation and stabilization of EZH2 by DCAF1/VprBP trigger aberrant gene silencing in colon cancer | ||
Mol Cell CRAMP1 drives linker histone expression to enable Polycomb repression
| ||
Mol Cell TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.
| ||
J Exp Clin Cancer Res EIF4A3-mediated biogenesis of circSTX6 promotes bladder cancer metastasis and cisplatin resistance | ||
Adv Sci (Weinh) The Interplay Between HIF-1α and EZH2 in Lung Cancer and Dual-Targeted Drug Therapy |
Reviews
What customers say
One reviewer (Nikhil Ghate, January 2022) gave this EZH2 antibody a 5-star rating for Western Blot. They used HCT116 whole cell extract at 20 µg/lane with a 1:1000 dilution, and included lanes showing transient expression of EZH2, confirming the antibody's specificity and performance in detecting both endogenous and overexpressed target protein.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nikhil (Verified Customer) (01-07-2022) | HCT116 whole cell extract, 20micro/lane. Lane 2-4 transient expression of EZH2
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