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  • KD/KO Validated

SUZ12 Polyclonal antibody

SUZ12 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, rat





Cat no : 20366-1-AP


ChET 9 protein, CHET9, JJAZ1, Joined to JAZF1 protein, KIAA0160, Polycomb protein SUZ12, SUZ12

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20366-1-AP targets SUZ12 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name suppressor of zeste 12 homolog (Drosophila)
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberNM_015355
Gene Symbol SUZ12
Gene ID (NCBI) 23512
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.


Product Specific Protocols
WB protocol for SUZ12 antibody 20366-1-APDownload protocol
IHC protocol for SUZ12 antibody 20366-1-APDownload protocol
IP protocol for SUZ12 antibody 20366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.

Authors - Iva A Tchasovnikarova
  • KD Validated


A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

Authors - Xu Wang

Eur J Med Chem

Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.

Authors - Cheng Wang

Sci Rep

Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.

Authors - Masamitsu N Asaka

Am J Cancer Res

N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex.

Authors - Jing Sun

Acta Pharmacol Sin

AZD9291 inactivates the PRC2 complex to mediate tumor growth inhibition.

Authors - Kai-Li Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Nikhil (Verified Customer) (01-07-2022)

HCT116 whole cell extract, 20micro/lane. Lane 2-4 transient expression of EZH2

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HCT116
SUZ12 Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:20366-1-AP)