Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
20366-1-AP targets SUZ12 in WB, IP, IHC, ELISA applications and shows reactivity with human, rat samples.
|Tested Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||suppressor of zeste 12 homolog (Drosophila)|
|Calculated molecular weight||83 kDa|
|Observed molecular weight||80-90 kDa|
|GenBank accession number||NM_015355|
|Gene ID (NCBI)||23512|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.
TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.
A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.
Eur J Med Chem
Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.
Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.
Am J Cancer Res
N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex.
Acta Pharmacol Sin
AZD9291 inactivates the PRC2 complex to mediate tumor growth inhibition.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Nikhil (Verified Customer) (01-07-2022)
HCT116 whole cell extract, 20micro/lane. Lane 2-4 transient expression of EZH2