Tested Applications
| Positive WB detected in | HEK-293 cells, HaCaT cells, fetal human brain tissue |
| Positive IF-P detected in | mouse brain tissue, rat brain tissue, Human dorsal cerebral organoid |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25265-1-AP targets Nesprin 2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | spectrin repeat containing, nuclear envelope 2 |
| Calculated Molecular Weight | 799 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | NM_015180 |
| Gene Symbol | Nesprin 2 |
| Gene ID (NCBI) | 23224 |
| RRID | AB_2879995 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Nesprin 2 antibody 25265-1-AP | Download protocol |
| WB protocol for Nesprin 2 antibody 25265-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Nesprin 2 (SYNE2) Polyclonal antibody, catalog number 25265-1-AP, has 1 total citation. It appears in the journal Cancer Letters (2024), in a study on SMARCAL1 inhibition in small cell lung cancer. The associated research field is oncology/cancer biology, with the antibody applied in Western blot (WB) for human samples.
| Species | Application | Title |
|---|---|---|
Cancer Lett Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer |
Reviews
What customers say
Both reviewers gave 5-star ratings, praising the antibody's performance for detecting Nesprin-2 (Syne-2). Used at a 1:500 dilution, it produced clear staining in human cerebral organoids, fetal brain tissue, and neural stem cells. Applications included immunofluorescence, immunohistochemistry, and cell culture. One reviewer imaged a dorsal organoid on a confocal microscope at 100x, highlighting crisp ventricular-like zone labeling. Overall, users report reliable, high-quality staining across multiple human brain-derived sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ryszard (Verified Customer) (09-02-2025) | Ventricular-like zone of a human brain dorsal organoid stained for Nesprin-2, imaged on an AXR Nikon Confocal Microscope 100x - max projection
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Ryszard (Verified Customer) (07-25-2022) | Very nice staining with the Syne-2 antibody. I tried on Human Fetal Brain Tissue, Human Cerebral Organoids and Human Neural Stem Cells. They are very nice. Images attached to this comment from a Human Cerebral Organoid (Staining of Nesprin-2 in white LUT)
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