Nesprin 2 Polyclonal antibody

Nesprin 2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 25265-1-AP


KIAA1011, Nesprin 2, NUA, NUANCE, Protein NUANCE, SYNE 2, SYNE2

Tested Applications

Positive WB detected inHEK-293 cells
Positive IF detected inHuman dorsal cerebral organoid, mouse brain tissue, rat brain tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25265-1-AP targets Nesprin 2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name spectrin repeat containing, nuclear envelope 2
Calculated Molecular Weight 799 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberNM_015180
Gene Symbol SYNE2
Gene ID (NCBI) 23224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.


Product Specific Protocols
WB protocol for Nesprin 2 antibody 25265-1-APDownload protocol
IF protocol for Nesprin 2 antibody 25265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Ryszard (Verified Customer) (07-25-2022)

Very nice staining with the Syne-2 antibody. I tried on Human Fetal Brain Tissue, Human Cerebral Organoids and Human Neural Stem Cells. They are very nice. Images attached to this comment from a Human Cerebral Organoid (Staining of Nesprin-2 in white LUT)

  • Applications: Immunohistochemistry, Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Cerebral Organoids/Human Fetal Brain Tissue and Neural Stem Cells
Nesprin 2 Antibody Immunohistochemistry,Immunofluorescence,Cell culture validation (1:500 dilution) in Human Cerebral Organoids/Human Fetal Brain Tissue and Neural Stem Cells (Cat no:25265-1-AP)