|Positive WB detected in||human liver tissue, mouse testis tissue, Y79 cells|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14511-1-AP targets Synaptotagmin-1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synaptotagmin-1 fusion protein Ag5970|
|Full Name||synaptotagmin I|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||65 kDa, 47 kDa|
|GenBank accession number||BC058917|
|Gene ID (NCBI)||6857|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.
|Product Specific Protocols|
|WB protocol for Synaptotagmin-1 antibody 14511-1-AP||Download protocol|
|IHC protocol for Synaptotagmin-1 antibody 14511-1-AP||Download protocol|
|IF protocol for Synaptotagmin-1 antibody 14511-1-AP||Download protocol|
|IP protocol for Synaptotagmin-1 antibody 14511-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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