Synaptotagmin-1 Polyclonal antibody

Synaptotagmin-1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14511-1-AP

Synonyms

SVP65, Synaptotagmin 1, synaptotagmin I, SYT, SYT1, SytI



Tested Applications

Positive WB detected inhuman liver tissue, mouse testis tissue, Y79 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14511-1-AP targets Synaptotagmin-1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptotagmin-1 fusion protein Ag5970
Full Name synaptotagmin I
Calculated molecular weight 48 kDa
Observed molecular weight 65 kDa, 47 kDa
GenBank accession numberBC058917
Gene symbol SYT1
Gene ID (NCBI) 6857
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-1 antibody 14511-1-APDownload protocol
IHC protocol for Synaptotagmin-1 antibody 14511-1-APDownload protocol
IF protocol for Synaptotagmin-1 antibody 14511-1-APDownload protocol
IP protocol for Synaptotagmin-1 antibody 14511-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochim Biophys Acta

Quantitative proteomics study of host response to virulent and attenuated pseudorabies virus infection in mouse brain.

Authors - Hao-Long Zeng
ratWB, IF

PLoS One

Identification of Protein Network Alterations upon Retinal Ischemia-Reperfusion Injury by Quantitative Proteomics Using a Rattus norvegicus Model.

Authors - Han Tian
mouseWB

J Proteomics

Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.

Authors - Hao-Long Zeng
mouseIHC,WB

Biochim Biophys Acta Mol Cell Biol Lipids

Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet.

Authors - Sara Sancho-Knapik

Front Mol Neurosci

A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13.

Authors - Yun Li
humanWB

Theranostics

ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells.

Authors - Tianhui Pan