Secondary Antibody Conjugates
Secondary-Antibody-Conjugates-AM112
Synonyms
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Overview
Active Motif's secondary antibody conjugates offer an improved method for the detection of primary antibodies. Having developed many quality primary antibodies, we found that the quality of secondary antibodies was also key to producing superior results. We therefore offer these secondary antibodies, conjugated to HRP for detecting primary antibodies bound to their targets. To conjugate dyes or other labels to primary antibodies and proteins, we offer our Sortag-IT™ Labeling Kits.
HeLa cells were either left untreated or treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117) and Chromeo 642 Goat anti-rabbit IgG, while tubulin was visualized using alpha Tubulin mouse mAb (Clone 5-B-1-2) and Chromeo 488 Goat anti-mouse IgG. The nuclei were counter-stained with DAPI, then the three separate images were merged.
Active Motif's fluorescent ATTO dyes and fluorescent Chromeo™ 488, Chromeo™ 505 and Chromeo™ 494 dyes have been certified by Leica Microsystems for STimulated Emission Depletion (STED) microscopy, as they exhibited superior staining when compared to other fluorescent antibody conjugates. Active Motif offers a range of Sortag-IT labeling Kits to conjugate these fluors to our AbFlex® recombinant antibodies.