SignalBright Plus Chemiluminescent Substrate


Mid to high femtogram

Signal duration:

Up to 24 hours


suitable for PVDF and nitrocellulose membranes

Cat no : PK10012

Product Information

SignalBright Plus is a luminol-based, chemiluminescent substrate for HRP (horseradish peroxidase) which provides a much higher sensitivity over traditional ECL reagents. SignalBright Plus provides mid to high- femtogram detection levels in Western Blot and ELISA analysis, with long lasting signal up to 24 hours. Signal intensity is up to 2X that of SignalBright Pro.

Key Characteristics:

· Sensitivity: Mid to high femtogram

· Signal duration: Up to 24 hours

· Compatibility: suitable for PVDF and nitrocellulose membranes

· X2 Signal intensity verses SignalBright Pro 

· Minimal optimization re quired

Instructions for Use

SignalBright Plus Detection Kit is prepared by combining a 1:1 ratio of Chemiluminescent Substrate Solution and  Stable Peroxide Solution  to create a working solution. Allow the working solution to come to room temperature before applying to the membrane, make sure this is done in darkness. Apply a minimum of 0.1ml of the working solution per membrane cm2. Leave 5 minutes for signal to develop prior to imaging.

· Recommended primary antibody concentration: 0.02-0.5 ug/ml

· Recommended HRP-conjugated secondary antibody concentration: 2-20 ng/ml

Product Information

PK100126mL 3mL Chemiluminescent Substrate Solution
3mL Stable Peroxide Solution
20mL10mL Chemiluminescent Substrate Solution
10mL Stable Peroxide Solution
100mL50mL Chemiluminescent Substrate Solution
50mL Stable Peroxide Solution
200mL100mL Chemiluminescent Substrate Solution
100mL Stable Peroxide Solution


Store at 4ºC. Expires 1 year after purchase.

Cited in Article as

PK10012, SignalBright Plus Chemiluminescent Substrate, Proteintech, IL, USA