SignalBright Pro Chemiluminescent Substrate


Mid to high femtogram

Signal duration:

More than 12 hours


suitable for PVDF and nitrocellulose membranes

Cat no : PK10011

Product Information

SignalBright Pro is a luminol-based, chemiluminescent substrate for HRP (horseradish peroxidase) which provides a much higher sensitivity over traditional ECL reagents. SignalBright Pro provides mid to high- femtogram detection levels in Western Blot and ELISA analysis, with long lasting signal up to 24 hours.

Key Characteristics:

· Sensitivity: Mid to high femtogram

· Signal duration: >12 hours

· Compatibility: suitable for PVDF and nitrocellulose membranes

· Comparable sensitivity to SuperSignal™ West Dura.

Instructions for Use

SignalBright Pro Chemiluminescent Detection is prepared by combining a 1:1 ratio of the Chemiluminescent Substrate Solution  and   Stable Peroxide Solution  to create a working solution. Allow the working solution to come to room temperature before applying to the membrane, make sure this is done in darkness. Apply a minimum of 0.1ml of the working solution per membrane cm2. Leave 5 minutes for signal to develop prior to imaging.

· Recommended primary antibody concentration: 0.02-1 ug/ml

· Recommended HRP-conjugated secondary antibody concentration: 4-20 ng/ml

Product Information

PK1001120mL10mL Chemiluminescent Substrate Solution
10mL Stable Peroxide Solution
100mL50mL Chemiluminescent Substrate Solution
50mL Stable Peroxide Solution


Store at 4ºC. Expires 1 year after purchase.

Cited in Article as

PK10011, SignalBright Pro Chemiluminescent Substrate, Proteintech, IL, USA