SignalBright Pro Chemiluminescent Substrate

Sensitivity:

Mid to high femtogram

Signal duration:

Up to 24 hours

Compatibility:

suitable for PVDF and nitrocellulose membranes

Cat no : PK10011



Instructions for Use

SignalBright Pro is a luminol-based, chemiluminescent substrate for HRP (horseradish peroxidase) which provides a much higher sensitivity over traditional ECL reagents. SignalBright Pro provides mid to high- femtogram detection levels in Western Blot and ELISA analysis, with long lasting signal up to 24 hours.

Key Characteristics:

· Sensitivity: Mid to high femtogram

· Signal duration: Up to 24 hours

· Compatibility: suitable for PVDF and nitrocellulose membranes

· Comparable sensitivity to SuperSignal™ West Dura.

SignalBright Pro Chemiluminescent Detection is prepared by combining a 1:1 ratio of the Chemiluminescent Substrate Solution  and   Stable Peroxide Solution  to create a working solution. Allow the working solution to come to room temperature before applying to the membrane, make sure this is done in darkness. Apply a minimum of 0.1ml of the working solution per membrane cm2. Leave 5 minutes for signal to develop prior to imaging.

· Recommended primary antibody concentration: 0.02-1 µg/ml

· Recommended HRP-conjugated secondary antibody concentration: 4-20 ng/ml

Product Information

Cat.SizeContent
PK100116mL 3mL Chemiluminescent Substrate Solution
3mL Stable Peroxide Solution
20mL10mL Chemiluminescent Substrate Solution
10mL Stable Peroxide Solution
100mL50mL Chemiluminescent Substrate Solution
50mL Stable Peroxide Solution
200mL100mL Chemiluminescent Substrate Solution
100mL Stable Peroxide Solution

Storage

Store at 4ºC. Expires 1 year after purchase.