SignalBright Pro Chemiluminescent Substrate

• 

  Serial dilutions of RUVBL primary antibody (10210-2-AP)
  Primary: Proteintech RUVBL1 (10210-2-AP) at various dilutions (see image)
  Secondary: Proteintech HRP-conjugated Affinipure Goat Anti-Rabbit IgG (SA00001-2); 1:6,000
  Exposure time: 1 min
  Chemiluminescent substrates from leading competitor, SignalBright Pro (PK10011), SignalBright Max (PK10013)

• 

  Serial dilutions of HEK-293 cell lysates
  Primary: Proteintech RUVBL1 (10210-2-AP); 1:8,000
  Secondary: Proteintech HRP-conjugated Affinipure Goat Anti-Rabbit IgG (SA00001-2); 1:6,000
  Exposure time: 1 min
  Chemiluminescent substrates from leading competitor, SignalBright Pro (PK10011), SignalBright Max (PK10013)

Cat No. PK10011

Publications

6

Sensitivity:

Mid to high femtogram

Signal duration:

More than 12 hours

Compatibility:

suitable for PVDF and nitrocellulose membranes

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Product Information

SignalBright Pro is a luminol-based, chemiluminescent substrate for HRP (horseradish peroxidase) which provides a much higher sensitivity over traditional ECL reagents. SignalBright Pro provides mid to high- femtogram detection levels in Western Blot and ELISA analysis, with long lasting signal up to 24 hours.

Key Characteristics:

· Sensitivity: Mid to high femtogram

· Signal duration: >12 hours

· Compatibility: suitable for PVDF and nitrocellulose membranes

· Comparable sensitivity to SuperSignal™ West Dura.

Instructions for Use

SignalBright Pro Chemiluminescent Detection is prepared by combining a 1:1 ratio of the Chemiluminescent Substrate Solution  and   Stable Peroxide Solution  to create a working solution. Allow the working solution to come to room temperature before applying to the membrane, make sure this is done in darkness. Apply a minimum of 0.1ml of the working solution per membrane cm2. Leave 5 minutes for signal to develop prior to imaging.

· Recommended primary antibody concentration: 0.02-1 ug/ml

· Recommended HRP-conjugated secondary antibody concentration: 4-20 ng/ml

Product Information

Cat.	Size	Content
PK10011	20mL	10mL Chemiluminescent Substrate Solution
		10mL Stable Peroxide Solution
	100mL	50mL Chemiluminescent Substrate Solution
		50mL Stable Peroxide Solution

Storage

Store at 4ºC. Expires 1 year after purchase.

Protocol

SignalBright Pro Chemiluminescent Substrate Protocol

Cited in Article as

PK10011, SignalBright Pro Chemiluminescent Substrate, Proteintech, IL, USA

Publications

Application	Title
WB	Front Cell Infect Microbiol; Activation of the Wnt signaling pathway and its role in epithelial-mesenchymal transition and hepatic fibrosis in alveolar echinococcosis; Authors - Binjie Wu
	Arch Microbiol; Identification and antibacterial activity of a novel antimicrobial peptide attacin from Conogethes punctiferalis; Authors - Yuhang Wang
	J Agric Food Chem; Allicin Attenuates Mitochondrial Calcium (Ca2+) Overload Induced by Acrylamide in Hepatocytes via the NAD+/SIRT3-FoxO3 Axis; Authors - Lu Li
	J Agric Food Chem; Diosmetin Alleviates Cerebral Ischemia/Reperfusion Injury by Modulating Tryptophan Metabolism through the Microbiota-Gut-Brain Axis; Authors - Mingwei Peng
WB	Int J Mol Sci; Anti-Her2 CAR-NK92 Cells and Their Exosomes: Generation, Characterization, and Selective Cytotoxicity Against Her2-Positive Tumor Cells.; Authors - Alexandru Tîrziu
	Am J Physiol Cell Physiol; Insulin potentiates mechanical responses in small dorsal root ganglion neurons by increasing the sensitization of TRPV4 channels; Authors - Ayumi Fukazawa