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Sp1 non-phospho antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Activ Motif logo

Cat No : 39809,39810 39809

Synonyms

Sp1, Sp-1, Sp 1, pAb, polyclonal, antibody, antibodies, transcription, transcriptional activation, specificity protein 1, dna damage, ATM, atm phospho ser1981, atr, double strand break, dsb, non-phospho, sample, dbdabs

Validation Data Gallery

  • Sp1 non-phospho antibody tested by Western blot. Detection of unphosphorylated Sp1 by Western blot. Whole-cell extract of U2OS cells (25 ug) probed with Sp1 non-phospho antibody at a dilution of 1:500. Lane 1: No treatment. Lane 2: Cells treated with H2O2.

    Sp1 non-phospho antibody tested by Western blot. Detection of unphosphorylated Sp1 by Western blot. Whole-cell extract of U2OS cells (25 ug) probed with Sp1 non-phospho antibody at a dilution of 1:500. Lane 1: No treatment. Lane 2: Cells treated with H2O2.

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB: 1:500 - 1:1,000 dilution
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This Sp1 antibody was raised against a peptide containing serine 101 of human Sp1.
Full Name Sp1 non-phospho antibody (pAb)
Synonyms Sp1, Sp-1, Sp 1, pAb, polyclonal, antibody, antibodies, transcription, transcriptional activation, specificity protein 1, dna damage, ATM, atm phospho ser1981, atr, double strand break, dsb, non-phospho, sample, dbdabs
Molecular weight 96 kDa
GenBank accession numberNP_612482
RRIDAB_2793349
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The Sp1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain. Sp1 contains two SQ/TQ domains, which are found in proteins phosphorylated by the DNA-damage response transducer/effector kinase ATM. Phosphorylation of serine 101 (dependent upon ATM and ATR) is stimulated by double strand DNA breakage (DSBs) as well as by blocks to DNA replication induced by treatment with hydroxyurea or UV irradiation. Sp1 phospho Ser101 co-localizes with ATM phospho Ser1981 at DSBs, suggesting that this phosphorylated version of Sp1 plays a role in double strand break repair.