TAF1-Specific Polyclonal antibody

TAF1-Specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20260-1-AP

Synonyms

BA2R, CCG1, CCGS, Cell cycle gene 1 protein, DYT3, KAT4, N TAF1, NSCL2, OF, P250, TAF(II)250, TAF1, TAF2A, TAFII 250, TAFII250, TBP associated factor 250 kDa



Tested Applications

Positive WB detected inmouse brain tissue, Jurkat cells
Positive IHC detected inhuman prostate cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

20260-1-AP targets TAF1-Specific in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
Calculated molecular weight 213 kDa
Observed molecular weight 50 kDa
GenBank accession numberNM_138923
Gene symbol TAF1
Gene ID (NCBI) 6872
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAF1, also named as BA2R, CCG1, CCGS, TAF2A, p250, Cell cycle gene 1 protein, TAFII250 and DYT3, belongs to the TAF1 family. TAF1 is the largest component and core scaffold of the TFIID basal transcription factor complex. It contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. TAF1 phosphorylates GTF2A1 and GTF2F1 on Ser residues. It possesses DNA-binding activity. It is essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3) which also called X-linked dystonia-parkinsonism (XDP). The antibody is specific to TAF1.

Protocols

Product Specific Protocols
WB protocol for TAF1-Specific antibody 20260-1-APDownload protocol
IHC protocol for TAF1-Specific antibody 20260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols